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目的探讨江苏地区慢性丙型肝炎患者血浆维生素D水平及维生素D受体(vitamin D receptor,VDR)基因多态性与丙型肝炎易感性的关系。方法选择97例慢性丙型肝炎患者和110例健康者为研究对象,采用酶联免疫吸附试验检测其血浆25-羟基维生素D(25(OH)D)水平,采用Taq Man技术对VDR基因(rs2228570和rs7975232位点)进行基因分型。结果慢性丙型肝炎患者血浆25(OH)D水平低于健康者,差异有统计学意义(Z=-6.175,P<0.001)。25(OH)D水平与丙氨酸氨基转移酶、天门冬氨酸氨基转移酶及谷氨酰转肽酶水平均呈负相关(均有P<0.05)。rs7975232位点的突变基因型AA与25(OH)D水平(F=3.719,P=0.026)及丙型肝炎易感性(调整OR=0.238,95%CI:0.074~0.765,P=0.016)均有统计学关联。结论慢性丙型肝炎患者易患维生素D缺乏,且维生素D水平与肝功能相关;VDR基因多态性可能与血浆25(OH)D水平及丙型肝炎易感性有关。
Objective To investigate the relationship between plasma vitamin D levels and vitamin D receptor (VDR) gene polymorphism and susceptibility to hepatitis C in patients with chronic hepatitis C in Jiangsu province. Methods Ninety-seven patients with chronic hepatitis C and 110 healthy subjects were enrolled in this study. The level of 25-hydroxyvitamin D (25 (OH) D) in plasma was detected by enzyme linked immunosorbent assay (ELISA), and the VDR gene (rs2228570 And rs7975232 sites). Results The plasma levels of 25 (OH) D in patients with chronic hepatitis C were significantly lower than those in healthy controls (Z = -6.175, P <0.001). The level of 25 (OH) D was negatively correlated with the levels of alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase (all P <0.05). The genotype AA and 25 (OH) D levels of rs7975232 (F = 3.719, P = 0.026) and susceptibility to hepatitis C (adjusted OR = 0.238,95% CI: 0.074-0.765, P = 0.016) Statistical correlation. Conclusions Patients with chronic hepatitis C are predisposed to vitamin D deficiency and the level of vitamin D is related to liver function. VDR gene polymorphism may be related to plasma 25 (OH) D level and the susceptibility to hepatitis C infection.