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目的探讨腹腔注射2-脱氧-D-葡萄糖(2-DG)能否激活大鼠下丘脑视上核(SON)和室旁核(PVN)神经元而表达Fos。方法健康雄性SD大鼠12只,随机分为腹腔注射2-DG组(6只)、生理盐水对照组(3只)及正常对照组(3只)。各自处理后,应用免疫组织化学方法,观察各组下丘脑SON和PVN内Fos表达及其与催产素(OT)和加压素(VP)的双标情况,同时采用ELISA方法对血清中OT和VP的含量进行检测。结果与生理盐水对照组和正常对照组相比,2-DG引发的特异性Fos免疫阳性产物主要集中分布于下丘脑外侧区和穹隆周区,在SON、PVN也有密集表达。SON和PVN内的Fos表达与该区的特异性神经活性物质OT和VP有共存。OT/Fos双标细胞率(双标细胞占OT阳性细胞的百分率)在SON和PVN分别为87.10%、90.57%,明显高于VP/Fos在这两个核团的双标率(双标细胞占VP阳性细胞的百分率,68.42%、76.92%),两者比较差异有统计学意义(P<0.05)。ELISA检测结果显示,2-DG组动物血清中OT和VP水平与对照组相比无明显变化。结论腹腔注射2-DG可激活大鼠下丘脑SON和PVN内OT和VP神经元表达Fos,SON和PVN可能参与2-DG诱导的急性应激反应。
Objective To investigate whether intraperitoneal injection of 2-deoxy-D-glucose (2-DG) can activate the hypothalamic supraoptic nucleus (SON) and paraventricular nucleus (PVN) neurons to express Fos. Methods Twelve healthy male Sprague-Dawley rats were randomly divided into 2-DG group (6), saline control group (3) and normal control group (3). After treatment, the expression of Fos in SON and PVN of hypothalamus and the double labeling of OT with vasopressin (VP) were observed by immunohistochemistry. Meanwhile, the serum levels of OT and VP content of the test. Results Compared with the normal saline group and the normal control group, the specific Fos immunopositive products induced by 2-DG were mainly distributed in the lateral hypothalamus and perinucleus area, and also strongly expressed in SON and PVN. Fos expression in SON and PVN coexisted with OT and VP, which are specific neurotransmitters in this region. The ratio of OT / Fos double-labeled cells (the percentage of double-labeled cells to OT-positive cells) was 87.10% and 90.57% in SON and PVN, respectively, which was significantly higher than that in VP / Fos Accounting for 68.42%, 76.92% of VP positive cells), the difference was statistically significant (P <0.05). The results of ELISA showed that the levels of OT and VP in serum of 2-DG group did not change significantly compared with the control group. Conclusion Intraperitoneal injection of 2-DG can activate Fos, SON and PVN in OT and VP neurons in SON and PVN of hypothalamus, which may be involved in 2-DG induced acute stress response.