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目的 探讨混苯对人体脂质过氧化的作用机制以及检测混苯作业人员早期的生物学指标 ,观察混苯暴露对人体白细胞计数、血红蛋白以及血清MDA、SOD、GSH Px的影响。方法 选择 133名长期从事喷漆作业的工人和 169名无接尘、接毒史的工人作为研究对象。同时将暴露组按苯浓度的不同分为高浓度组和低浓度组 ,用常规方法进行红蛋白和白细胞计数 ,测定血清中MDA含量和SOD、GSH Px活力。对结果分别进行方差分析和非参数统计 (F检验和H检验 )以及相关性分析等。结果 暴露组和对照组间白细胞计数、血红蛋白含量差别有显著性 (P <0 0 1) ,但白细胞计数、血红蛋白的异常检出率在两组间的差别无显著性。MDA含量及其异常检出率在两组间差别均有显著性 (P <0 0 1) ,且随着工龄的增长 ,暴露组MDA含量明显增加 (P <0 0 5)。与对照组相比 ,混苯组SOD活性及异常检出率明显增高 ,而GSH Px活性和异常检出率却显著下降 ,差别均有显著性 (P <0 0 1)。MDA含量与白细胞计数和GSH Px活性呈负相关 ,而与SOD活性呈正相关。结论 长期接触低浓度混苯 ,将导致MDA含量和SOD活性增加 ,GSH Px活性下降 ,且改变出现在白细胞改变之前。可以认为MDA含量以及抗氧化酶类 (如SOD、GSH Px等 )的活性可作为混苯作业人员的早期生物学监测指标。
Objective To investigate the mechanism of mixed benzene on human lipid peroxidation and to detect the early biological indicators of mixed benzene workers to observe the effects of mixed benzene on human white blood cell count, hemoglobin and serum MDA, SOD, GSH Px. Methods 133 long-term painters and 169 workers without exposure to dust and poisoned drug were selected as research objects. At the same time, the exposure group was divided into high concentration group and low concentration group according to the benzene concentration, and the content of serum MDA and SOD, GSH Px were measured by routine methods. The results of variance analysis and nonparametric statistics (F test and H test) and correlation analysis. Results There was a significant difference in white blood cell count and hemoglobin between the exposed group and the control group (P <0.01). However, there was no significant difference in the detection rates of white blood cell count and hemoglobin between the two groups. The content of MDA and the detection rate of abnormality in the two groups were significantly different (P <0.01). With the increase of seniority, MDA content in the exposed group increased significantly (P <0.05). Compared with the control group, the SOD activity and abnormal detection rate in mixed benzene group were significantly increased, while the activity and abnormal detection rate of GSH Px were significantly decreased, the difference was significant (P <0.01). MDA content was negatively correlated with white blood cell count and GSH Px activity, but positively correlated with SOD activity. Conclusion Long-term exposure to mixed benzene at low concentrations will result in an increase in MDA content and SOD activity, and a decrease in GSH Px activity, with changes occurring before leukocyte alteration. It is believed that the activity of MDA and antioxidant enzymes (such as SOD, GSH Px, etc.) can be used as early biological monitoring indicators for mixed benzene workers.