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目的观察内源性一氧化氮(nitric ox ide,NO)对裸鼠肝癌模型中组蛋白去乙酰化酶4(histone deacety lase4,HDAC4)表达的影响,从而探讨新的抗肿瘤思路。方法免疫组化方法检测已经建立好的裸鼠肝癌模型中对照组、5-氟尿嘧啶组(5-FU)、5-氟尿嘧啶+L-精氨酸组(5-FU+L-A rg)诱导型一氧化氮合酶(inducib le nitric ox idesynthase,iNO S)与HDAC4的表达情况。结果 iNO S在对照组、5-FU组、5-FU+L-A rg组中表达呈平行递增关系,而HDAC4则表达为下降关系,每两组间比较差异均有统计学意义(P<0.05),iNO S和HDAC4在5-FU组、5-FU+L-A rg组的表达有显著相关性(P<0.05)。结论内源性NO下调HDAC4的表达,是HDAC功能作用的重要调节者,为抗肿瘤研究提供新的思路。
Objective To investigate the effect of endogenous nitric oxide (NO) on the expression of histone deacetylase 4 (HDAC4) in nude mice with hepatocellular carcinoma (HCC) and to explore new antitumor strategies. Methods Immunohistochemistry was used to detect the expression of 5-FU, 5-fluorouracil + L-arginine (5-FU + LA rg) Expression of nitric oxide synthase (iNOS) and HDAC4 in rats. Results The expression of iNO S in the control group, the 5-FU group and the 5-FU + LA rg group showed a parallel and increasing relationship, while the expression of HDAC4 was decreased. The difference between the two groups was statistically significant (P0.05) , iNO S and HDAC4 in 5-FU group and 5-FU + LA rg group were significantly correlated (P <0.05). Conclusion Endogenous NO down-regulates the expression of HDAC4, which is an important regulator of HDAC function and provides a new idea for anti-tumor research.