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采用培养皿法和常规生化方法,研究了新型除草剂唑嘧氯草胺(暂定名,代号:ZJ-2725)的作用机制。结果显示,同时添加20mg/L浓度的缬氨酸、亮氨酸和异亮氨酸能完全恢复唑嘧氯草胺对苘麻芽的生长抑制作用,而添加相同浓度的单一支链氨基酸只能部分消除其抑制作用。离体条件下,随着唑嘧氯草胺浓度的增大,对乙酰乳酸合成酶(ALS)活性的抑制率增加,ALS活性随反应时间的延长而增加,且两者之间符合Michaelis-Meten方程;活体条件下,唑嘧氯草胺对苘麻的ALS也表现出明显的抑制作用,ALS活性明显下降。表明唑嘧氯草胺在植物体内抑制了3种支链氨基酸的生物合成,进而导致植物蛋白质合成受阻而使植物生长受到抑制,ALS即为唑嘧氯草胺的作用靶标。
The mechanism of action of the new herbicide azacilimid (tentative name, code: ZJ-2725) was studied by petri dish method and conventional biochemical methods. The results showed that addition of valine, leucine and isoleucine at a concentration of 20 mg / L could completely restrain the growth inhibition of geranium buds by gemcitabine, whereas adding the same concentration of single branched-chain amino acids only Partially eliminate its inhibitory effect. In vitro, the inhibitory rate of acetolactate synthase (ALS) activity increased with the concentration of azocopyram, and the activity of ALS increased with the increase of reaction time, and the two met the Michaelis-Meten In vivo, azacyprol was also found to significantly inhibit the ALS activity of Cannabina maxima and the ALS activity was significantly decreased. It is indicated that azacil and chlorimuron suppresses the biosynthesis of three kinds of branched-chain amino acids in plants, which leads to the inhibition of plant protein synthesis and the inhibition of plant growth. ALS is the target of azacil and acetochlor.