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目的采用R值综合评价法多指标综合评价调补肺肾三法(补肺健脾、补肺益肾、益气滋肾)对慢性阻塞性肺疾病(COPD)稳定期大鼠肺胶原和蛋白酶的调节作用。方法采用香烟熏吸联合细菌感染法制备COPD稳定期大鼠模型,于实验第9周起分别给予补肺健脾方、补肺益肾方、益气滋肾方及氨茶碱灌胃治疗至第20周,于实验第20、32周检测大鼠肺胶原和蛋白酶的9个指标,包括Ⅰ型胶原、Ⅲ型胶原、Ⅳ型胶原、基质金属蛋白酶-2(MMP-2)积分光密度(IOD)、基质金属蛋白酶-9(MMP-9)IOD、金属蛋白酶组织抑制剂-1(TIMP-1)IOD,MMP-2 mRNA、MMP-9 mRNA、TIMP-1 mRNA。建立R值综合评价法,并以此对肺胶原和蛋白酶的调节作用进行综合评价。结果第20、32周及综合20、32周,调补肺肾三法和氨茶碱对肺胶原和蛋白酶综合9指标的纠正强度显著(P<0.01)。第20、32周,综合纠正强度依次为补肺健脾、补肺益肾、益气滋肾、氨茶碱,且第32周补肺健脾、补肺益肾的纠正强度较氨茶碱显著,补肺益肾的纠正强度较本组第20周显著(P<0.05)。综合20、32周,综合纠正强度依次为补肺健脾、补肺益肾、益气滋肾、氨茶碱,且补肺健脾和补肺益肾纠正强度较氨茶碱显著(P<0.05或P<0.05)。结论调补肺肾三法对COPD稳定期大鼠肺胶原和蛋白酶的综合调节作用显著,且具有较好的远后效应,以补肺健脾、补肺益肾效果更佳。
Objective To evaluate the effects of Bufei Jianpi, Bufei Yishen, Yiqi Zishen on the pulmonary collagen and protease in rats with chronic obstructive pulmonary disease (COPD) The regulatory role. Methods COPD stable rat model was prepared by cigarette smoking combined with bacterial infection. From the 9th week of the experiment, rats were treated with Bufei Jianpi Fang, Bufei Yishen Recipe, Yiqi Zishen Recipe and aminophylline respectively At week 20, nine indexes of rat lung collagen and protease were detected at weeks 20 and 32 of the experiment, including type I collagen, type III collagen, type IV collagen, matrix metalloproteinase-2 (MMP-2) integral optical density IOD, MMP-9, IOD, TIMP-1, MMP-2 mRNA, MMP-9 mRNA and TIMP-1 mRNA. The comprehensive evaluation method of R value was established and the regulation of lung collagen and protease was evaluated comprehensively. Results After 20 weeks, 32 weeks and 20 weeks and 32 weeks, the corrective strength of lung and kidney three methods and aminophylline on lung collagen and protease comprehensive 9 indexes were significantly improved (P <0.01). The corrective intensity at the 20th and 32nd week was Bufei Jianpi, Bufei Yishen, Yiqi Zishen and aminophylline, and the corrective intensity of Bufei Yishen in the 32nd week was higher than that of aminophylline Significantly, the corrective strength of Bufei Yishen was significantly higher than that of the first 20 weeks (P <0.05). Comprehensive 20,32 weeks, the comprehensive correction intensity followed by Bufei Jianpi, Bufei Yishen, Yiqi Zishen, aminophylline, and Bufei Jianpi and Bufei Yishen correct intensity than aminophylline significantly (P < 0.05 or P <0.05). Conclusion tonifying the lung and kidney three methods of COPD stable rats lung collagen and protease comprehensive regulation of significant effect, and has a good long-term effect, to Bufei Jianpi, Bufei Yishen better.