采用高效液相色谱法,以乙腈和水为流动相,选用ZORBAX SB-C_(18)反相柱和紫外可变波长检测器,对15%阿维·螺虫悬浮剂中有效成分进行分离和定量分析。结果表明,阿维菌素和螺虫乙酯标准偏差分别为0.084和0.180,变异系数分别为2.69%和1.46%,平均回收率分别为99.58%和99.72%。该方法操作简便,分离效果好,精密度与准确度高,可同时快速测定阿维菌素和螺虫乙酯含量。 High performance liquid chromatography (HPLC) with acetonitrile and water as the mobile phase, ZORBAX SB-C 18 reversed-phase column and ultraviolet variable wavelength detector were used to separate and purify the active ingredients in 15% Quantitative analysis. The results showed that the standard deviations of abamectin and spirotetramat were 0.084 and 0.180, respectively. The coefficients of variation were 2.69% and 1.46% respectively. The average recoveries were 99.58% and 99.72% respectively. The method has the advantages of simple operation, good separation effect, high precision and high accuracy, and rapid and simultaneous determination of avermectin and spirotetramat ethyl.