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目的 观察艾滋病病毒外膜蛋白、核心蛋白与干扰素 (HIV -lenv/IFNα- 2b、HIV - 1gag/IFNα - 2b)融合基因的表达产物 ,能否作为理想的基因疫苗 ,使机体产生体液免疫和细胞免疫应答。方法 以小鼠为实验对象 ,用重组病毒vJ16env/IFNα - 2b、vJ38gag/IFNα - 2b免疫小鼠 ,以生理盐水和野生型痘苗病毒为对照组 ,检测小鼠外周血与脾淋巴细胞对ConA、Lps的反应性。用间接ELISA试验检测了重组病毒诱导小鼠产生的HIV -lenv/IFNα - 2b、HIV -lgag/IFNα - 2b抗体 ,用流式细胞仪测定小鼠脾细胞CD+ 4 、CD+ 8T细胞计数。结果 外周血与脾淋巴细胞对ConA、Lps的反应性 ,实验组与对照组比较差异有显著性意义 (P <0 0 5 )。vJ16env/IFNα - 2b与vJ38gag/IFNα - 2b能激发小鼠体内产生高滴度的抗体。CD+ 4 、T淋巴细胞与对照组比较差异有显著性意义 (P <0 0 5 )。CD+ 8T淋巴细胞与对照组比较无差异性 ,但呈增高趋势。结论 重组痘苗病毒能诱导小鼠产生较强的体液免疫和细胞免疫。重组痘苗病毒能在体外与HIV -lenv和HIV - 1gag阳性血清发生特异性反应 ,具有免疫原性和免疫反应性。
Objective To observe the expression products of HIV-1 envelope protein, core protein and interferon (HIV -lenv / IFNα-2b, HIV-1gag / IFNα-2b) fusion gene and to make it an ideal gene vaccine for humoral immunity and Cellular immune response. Methods Mice were immunized with recombinant virus vJ16env / IFNα - 2b and vJ38gag / IFNα - 2b. The normal saline and wild type vaccinia virus were used as control group to detect the effect of ConA, Lps reactivity. The HIV - lvv / IFNα - 2b and HIV - lgag / IFNα - 2b antibodies induced by recombinant virus were detected by indirect ELISA. The count of CD + 4 and CD + 8T cells in splenocytes of mice was determined by flow cytometry. Results The reactivity of ConA and LPS between peripheral blood and splenic lymphocytes was significantly different between the experimental group and the control group (P <0.05). vJ16env / IFNα - 2b and vJ38gag / IFNα - 2b stimulated mice to produce high titers of antibodies. CD + 4, T lymphocytes compared with the control group had significant difference (P <0 05). CD + 8T lymphocytes compared with the control group no difference, but showed an increasing trend. Conclusion Recombinant vaccinia virus can induce strong humoral and cellular immunity in mice. Recombinant vaccinia virus can react specifically with HIV-llenv and HIV-1gag-positive sera in vitro with immunogenicity and immunoreactivity.