论文部分内容阅读
目的探索水面血吸虫尾蚴富集方法,并与已有疫水检测技术相结合,建立新型快速敏感的疫水检测方法。方法选取大豆油、汽油、试剂煤油、异佛尔酮等进行扩展剂筛选。借助扩展剂在水面扩散时所形成的推力,对水面漂浮的尾蚴进行富集,然后采用PE吸附膜及C-6膜粘附富集的尾蚴,快速确定水体的感染性。同时探索扩展剂剂量与扩散半径之间的关系。结果汽油、试剂煤油、异佛尔酮均可作为扩展剂进行实验,异佛尔酮的扩散效果最优。采用扩展剂富集后进行吸附膜粘取,可显著提高水中尾蚴检出率。结论新方法可以有效提高感染性疫水的检出率,适合低感染度水体检测。
Objective To explore the method of enriching cercariae on the surface of Schistosoma japonicum and to establish a new rapid and sensitive method for detection of water ingestion by combining with the existing water detection techniques. Methods Soybean oil, gasoline, kerosene kerosene, isophorone and so on were selected for screening. With the expansion force of the spreading agent on the water surface, the cercariae floating on the water surface are enriched, and then the PE-absorbing membrane and C-6 membrane are used to adhere the enriched cercariae to quickly determine the water infectivity. At the same time to explore the relationship between the dose of diffusion agent and the diffusion radius. Results gasoline, kerosene reagent, isophorone can be used as a scavenger experiments, isophorone diffusion effect of the best. Adopting expander to enrich the adsorption film adhesion, can significantly improve the water cercariae detection rate. Conclusion The new method can effectively improve the detection rate of infectious water and is suitable for the detection of low-infecting water.