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利用Clark导入到红丰11为背景的回交导入系进行萌芽期和苗期的连续性耐旱鉴定,经萌芽期鉴定,获得36个耐旱定向选择导入系。对耐旱选择导入系进行全基因组SSR标记扫描,并以随机群体作为对照,计算供体基因型的导入频率,利用卡方测验检测显著偏分离的SSR标记位点,并结合GGT图示基因型软件对各染色体连锁群进行分析。其中在L连锁群的Satt398和Satt156两个位点有显著的供体片段“超导入”现象,供体基因型导入频率为0.916 7和0.958 3,卡方值高达182和201.5。另外,在F连锁群的Satt423,K连锁群的Satt167以及N连锁群的Sat_084等位点也出现供体导入片段的偏分离现象。同时,对萌芽期耐旱鉴定中相对发芽率表现超亲的35个株系采用性状-标记间的单向方差分析(P<0.01)和QTL定位,共检测到14个控制相对发芽率的QTL,分布在4个连锁群上,其中与卡方测验检测的结果相比较,在Satt156,Satt423,Sattt167等位点具有一致性,说明这些位点是与大豆耐旱性紧密相关的QTL位点。以上结果为进一步开展大豆耐旱性有利基因的精细定位、克隆和分子设计育种奠定了基础。
Using Clark introduced into Hongfeng 11 as the background backcross inbred line, germination and seedling stages of drought tolerance were identified, and 36 drought-tolerant selection lines were obtained after germination. Genome-wide SSR markers were screened in the drought-tolerant selection-introducing line, and the random population was used as a control to calculate the frequency of donor genotypes. Significantly segregated SSR markers were detected by the chi-square test. Combined with GGT-based genotypes The software analyzes each chromosome linkage group. Among them, there were significant “superconducting” donor sites in Satt398 and Satt156 loci of L linkage group. The frequencies of donor genotypes were 0.916 7 and 0.958 3, and the chi square values were as high as 182 and 201.5. In addition, Satt423 in F linkage group, Satt167 in linkage group K and Sat_084 in N linkage group also showed partial segregation of donor-introduced fragments. At the same time, 35 lines with relative germination rate of germplasm in germination identification were analyzed by one-way ANOVA (P <0.01) and QTL mapping, and a total of 14 QTLs controlling relative germination rate , Which were distributed on four linkage groups. Among them, compared with the results of chi-square test, there was consistency in Satt156, Satt423 and Sattt167, which indicated that these loci were closely related QTLs for soybean drought tolerance. The above results laid a foundation for further fine positioning, cloning and molecular design breeding of favorable genes for drought tolerance in soybean.