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用~(14)C 标记的 B_9水溶液涂布在苹果树新稍叶片,经显微放射自显影技术查明,B_9进入树体各器官比较迅速,处理后6小时,在茎、叶和芽中均有~(14)C—B,踪迹。其积累量随时间的增加而逐渐增多。涂布枝的上下、左右,非涂布枝的茎和叶脉维管束中有较多的分布,且在较长时期内始终有增无减,表明茎、叶脉维管束是~(14)C—B_9在树体内进行往返运转的主要通道。这种运转是一种非极性的多方向的运输。~(14)C—B_9主要是分布积累在茎内形成层,叶内栅栏组织、海绵组织、顶芽、腋芽的初生、次生分生组织和已分化的小叶中。作者认为,这种多部位的分布积累状况,与 B_9对苹果树营养生长的抑制及生殖生长的促进等调节机制存在密切关系。
The fresh leaves of apple tree were coated with ~ (14) C-labeled B_9 aqueous solution. The micro-autoradiography indicated that B_9 entered the organs of the tree more rapidly. After 6 hours of treatment, the stems, leaves and buds had ~ (14) C-B, traces. Its accumulation gradually increases with time. The branches and veins in the top and bottom, left and right, non-coated branches had more distribution in the vascular bundles, and there was always increasing in the longer period, indicating that the vascular bundles in the stem and vein were ~ (14) C- B_9 The main passage for round-trip operation in the tree. This operation is a non-polar multi-directional transport. ~ (14) C-B_9 mainly distributed in the stem formation layer, leaf palisade tissue, sponge tissue, apical bud, axillary bud primary and secondary meristematic tissue and differentiated leaflets. The author believes that the accumulation and distribution of this multi-site status is closely related to the regulatory mechanism of B_9 on the inhibition of vegetative growth and promotion of reproductive growth in apple trees.