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-观察了新鲜分离和经体外培养 36h的人外周血树突状细胞DC 0和DC 36 ,在GM CSF或TNF作用下 ,辅助淋巴因子和PHA激活的杀伤细胞 (LPAK )体外杀伤人肝癌细胞株BEL 740 2的影响。实验在BEL 740 2、LPAK和DC (DC 0或DC 36 )相混合的基本条件下 ,分别添加GM CSF (5 0 0u/ml,10 0u/ml)或TNF (5 0 0 0u/ml,5 0 0u/ml) ,温育 48h后用中性红比色法检测细胞毒活性。结果显示 ,加入GM CSF各组的细胞毒活性明显增高 (P <0 0 1) ,并在浓度为 10 0u/ml时达到最大效应。加入TNF各组的细胞毒活性也明显增高 (P <0 0 1) ,但存在着剂量依赖关系。DC 36各组与相应的DC 0组比较 ,细胞毒活性明显增高 (P <0 0 1)。表明新鲜分离的DC经 36h培养后 ,其辅助的抗肿瘤活性明显增强 ,与GM CSF和TNF也有更大的协同作用。
- Observed human peripheral blood dendritic cells DC 0 and DC 36 that were isolated and cultured in vitro for 36 hours. Under the action of GM CSF or TNF, adjuvant lymphokines and PHA-activated killer cells (LPAK) killed human hepatoma cell lines in vitro. The impact of BEL 740 2 Under the basic conditions of mixing BEL 740 2, LPAK and DC (DC 0 or DC 36), add GM CSF (500u/ml, 100u/ml) or TNF (500u/ml, respectively). 0 0u/ml), 48h after incubation, cytotoxic activity was measured by neutral red colorimetry. The results showed that the cytotoxic activity of the GM CSF group was significantly higher (P < 0.01), and the maximum effect was achieved at a concentration of 100 u/ml. The cytotoxic activity of each group added with TNF was also significantly higher (P < 0.01), but there was a dose-dependent relationship. The cytotoxic activity of DC 36 was significantly higher in each group than in the corresponding DC 0 group (P < 0 01). The results showed that the freshly isolated DCs were significantly more potent anti-tumor activity after 36 h incubation, and they also had a greater synergistic effect with GM CSF and TNF.