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目的:检测转录激活因子3(ATF3)及其靶基因Cyclin D1与Maspin在结直肠癌组织芯片中的表达,探讨ATF3表达在结直肠癌发生、发展中的意义。方法:构建含结直肠癌及癌旁相对正常肠黏膜的组织芯片,应用免疫组织化学检测ATF3、Cyclin D1及Maspin的表达情况。结果:ATF3、Cyclin D1与Maspin在结直肠癌中阳性表达率分别为70.3%、51.4%和59.5%,明显高于癌旁正常组织的21.6%、2.7%和13.5%,差异有统计学意义,χ2值分别为35.252、44.411和33.689,P值均为0.000;ATF3与结直肠癌患者的性别、年龄、肿瘤部位、肿瘤大小及肿瘤分化程度无关,而与浸润程度和淋巴结有无转移有关,浆膜层表达(83.3%)高于肌层(38.9%),有淋巴结转移(92.3%)高于无淋巴结转移(45.7%),χ2值分别为13.290和17.110,P值均为0.000;Cyclin D1与结直肠癌患者的性别、年龄、肿瘤部位及肿瘤大小无关,而与病理学分级、浸润程度和淋巴结有无转移有关,低分化组织表达(71.4%)高于高中分化组织(39.1%),浆膜层表达(61.1%)高于肌层(28.8%),有淋巴结转移(69.2%)高于无淋巴结转移(31.4%),χ2值分别为7.268、6.019和10.550,P值分别为0.007、0.014和0.001;Maspin与结直肠癌患者的性别、年龄、肿瘤部位、肿瘤大小、肿瘤分化程度、浸润深度及有无淋巴结转移均无关,P值均>0.05。ATF3、Cyclin D1与Maspin在结直肠癌中均高表达,ATF3与Cyc-lin D1在结直肠癌中表达呈正相关(r=0.362,P=0.000),与Maspin表达无明显相关(r=0.075,P=0.513)。结论:ATF3表达与结直肠癌的发生密切相关,ATF3可能通过调控Cyclin D1表达而促进结直肠癌的发生发展及侵袭转移。
OBJECTIVE: To detect the expression of ATF3, its target genes Cyclin D1 and Maspin in colorectal cancer tissue microarray, and to explore the significance of ATF3 expression in the development and progression of colorectal cancer. Methods: Tissue microarrays containing colorectal cancer and adjacent normal mucosa were constructed. The expressions of ATF3, Cyclin D1 and Maspin were detected by immunohistochemistry. Results: The positive rates of ATF3, Cyclin D1 and Maspin in colorectal cancer were 70.3%, 51.4% and 59.5%, respectively, which were significantly higher than those in 21.6%, 2.7% and 13.5% χ2 values were 35.252,44.411 and 33.689, P values were 0.000; ATF3 and colorectal cancer patients with gender, age, tumor location, tumor size and the degree of tumor differentiation has nothing to do with the degree of invasion and lymph node metastasis, pulp (83.3%) was higher than muscularis (38.9%), lymph node metastasis (92.3%) was higher than that without lymph node metastasis (45.7%), χ2 values were 13.290 and 17.110, P values were 0.000; Cyclin D1 and Colorectal cancer patients with gender, age, tumor location and tumor size has nothing to do with the pathological grade, degree of infiltration and lymph node metastasis, poorly differentiated tissue expression (71.4%) higher than high differentiated tissue (39.1%), (61.1%) was higher than that of muscularis (28.8%), lymph node metastasis (69.2%) was higher than that without lymph node metastasis (31.4%), the values of χ2 were 7.268,6.019 and 10.550, P values were 0.007,0.014 And 0.001; Maspin and colorectal cancer patients’ gender, age, tumor location, tumor size, tumor score Degree, depth of invasion and lymph node metastasis unrelated, P> 0.05. ATF3, Cyclin D1 and Maspin were all highly expressed in colorectal cancer. There was a positive correlation between ATF3 and Cyclin D1 expression in colorectal cancer (r = 0.362, P = 0.000), but no significant correlation with Maspin expression (r = 0.075, P = 0.513). CONCLUSION: ATF3 expression is closely related to the occurrence of colorectal cancer. ATF3 may promote the development, invasion and metastasis of colorectal cancer by regulating the expression of Cyclin D1.