对比了HPLC-RID法和UPLC-ELSD法对果糖、葡萄糖、蔗糖和麦芽糖4种可溶性糖的检测。结果表明:UPLC-ELSD法的分离效果、重复性、灵敏度等比HPLC-RID法好,确定了UPLC-ELSD法测定4种糖含量的技术参数,即流动相为0.2%(w/v)三乙胺溶于75%(v/v)乙腈,流速为0.2 m L/min,柱温为35℃,上样量为2μL,在6 min内可以灵敏、快速地检测出果糖等4种可溶性糖。利用UPLC-ELSD法测定玉米种子吸胀过程中蔗糖、麦芽糖、葡萄糖和果糖4种可溶性糖的含量,结果表明:胚乳在吸胀之前,仅检出蔗糖、葡萄糖和果糖,其中蔗糖含量最高,随着种子吸胀,蔗糖含量先下降后上升,而葡萄糖和果糖逐渐增加,吸胀48 h时检出麦芽糖。胚在吸胀之前,4种可溶性糖均可以检测到,且含量均显著高于胚乳,在吸胀过程中4种糖的变化规律与胚乳相似。因此,在玉米种子吸胀过程中,可能先动员蔗糖分解成葡萄糖和果糖,再动员贮藏物质合成蔗糖、麦芽糖、葡萄糖和果糖,为种子萌发生长提供能量和中间物质。 The HPLC-RID and UPLC-ELSD methods were used to detect the four kinds of soluble sugars of fructose, glucose, sucrose and maltose. The results showed that the separation, repeatability and sensitivity of UPLC-ELSD method were better than those of HPLC-RID method, and the technical parameters of UPLC-ELSD for determination of four kinds of sugar content were determined. The mobile phase was 0.2% (w / v) Ethylamine was dissolved in 75% (v / v) acetonitrile at a flow rate of 0.2 mL / min, the column temperature was 35 ℃, and the sample volume was 2 μL. The soluble and stable sugars . UPLC-ELSD was used to determine the contents of four kinds of soluble sugars such as sucrose, maltose, glucose and fructose in the process of imbibition. The results showed that only sucrose, glucose and fructose were detected in endosperm, and the content of sucrose was the highest Seeds dilatancy, sucrose content decreased first and then increased, while glucose and fructose gradually increased, detected 48h absorption of maltose. Four types of soluble sugars could be detected before the imbibation, and the contents of all four kinds of sugars were significantly higher than that of the endosperm. The changes of the four kinds of sugars were similar to the endosperm during the imbibition. Therefore, during the process of corn seed dilatation, it may first mobilize sucrose to decompose glucose and fructose, and then mobilize the stored materials to synthesize sucrose, maltose, glucose and fructose to provide energy and intermediates for seed germination and growth.