免疫组化染色技术已成为现代病理诊断中不可或缺的检测手段,特别是在判断肿瘤组织的起源、分类以及良、恶性肿瘤的鉴别等方面发挥着非常重要的作用。由于甲醛固定使标本的氨基酸残基分子之间或分子之间形成醛键,抗原决定簇被掩盖或破坏,使部分抗原-抗体反应不能顺利进行~([5])。因此,充分的抗原修复是染色效果好坏的重要环节。各单位都 Immunohistochemical staining technique has become an indispensable means of detection in modern pathological diagnosis, especially playing an important role in judging the origin, classification of tumor tissues and the identification of benign and malignant tumors. Due to the formaldehyde fixation, the antigenic determinants are masked or destroyed by the formation of aldehyde bonds between molecules or molecules of amino acid residues in the specimen, so that part of the antigen-antibody reaction can not be carried out smoothly (5). Therefore, the full antigen repair is an important part of the staining effect is good or bad. All units are