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目的 研究碱性成纤维细胞生长因子 (bFGF)对于纯化后的嗅黏膜成鞘细胞 (OECs)增殖的促进作用 ,探讨嗅黏膜OECs的增殖特性。 方法 应用差速贴壁和酶消化法对原代培养的嗅黏膜OECs进行纯化 ,用BrdU掺入法观察bFGF对嗅黏膜OECs增殖率的影响。 结果 在没有特定刺激因子作用时 ,嗅黏膜OECs本身有一定的增殖率 ,而加入 10 μg/L的bFGF对嗅黏膜OECs有一定的促增殖作用。 结论 在体外培养条件下 ,bFGF能提高嗅黏膜OECs的增殖率。
Objective To investigate the effects of basic fibroblast growth factor (bFGF) on the proliferation of purified olfactory ensheathing cells (OECs) and to investigate the proliferation of OECs. Methods OECs from primary cultured olfactory mucosa were purified by differential adherence and enzymatic digestion. BrdU incorporation was used to observe the effect of bFGF on the proliferation of OECs. Results OECs of the olfactory mucosa had a certain proliferation rate in the absence of specific stimulating factors, while 10 μg / L of bFGF had some effects on proliferation of OECs in the olfactory mucosa. Conclusion Under the conditions of in vitro culture, bFGF can increase the proliferation rate of OECs in olfactory mucosa.