论文部分内容阅读
以橡胶树热研88-13品种的幼嫩种子的珠心组织为材料,诱导出胚性愈伤组织。对胚性愈伤组织诱导培养基、继代培养基中不同的影响因素如植物生长调节剂的种类和浓度、钙离子浓度等进行了研究,筛选到了合适的影响因素。经过连续5个月的继代选择培养,逐渐诱导出易碎的胚性愈伤组织。对易碎胚性愈伤组织进行了长期继代培养。组织学切片证明长期继代培养的愈伤组织维持了胚性的状态。取继代培养了2年多的橡胶树热研88-13品种的珠心易碎胚性愈伤组织诱导胚状体,得到了186个胚状体,正在诱导植株再生。
The embryo callus was induced by using the tender heart tissue of the young seed of rubber tree hot research 88-13. In embryogenic callus induction medium, different factors in subculture medium such as plant growth regulators, such as the type and concentration of calcium ion concentration were studied, screened the appropriate factors. After five consecutive months of subculture selection, gradual induction of friable embryogenic callus. Long-term subculture of fragile embryogenic callus was performed. Histological sections proved that long-term subcultured callus maintained embryogenic status. Embryogenic embryos were induced from the embryogenic calluses of the embryo culture of the embryogenic calli 88-13, which had been subcultured for more than two years. A total of 186 embryoid bodies were obtained and plant regeneration was being induced.