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目的:通过紫外-可见分光光度法与高效液相色谱法对萘酚喹的含量测定进行对比,为建立萘酚喹的定量方法提供依据。方法:UV法以0.05 mol.L-1盐酸溶解样品,在341 nm处测定;HPLC色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相乙腈-0.25%二乙胺溶液(pH 2.5)(23∶77),流速0.8 mL.min-1,检测波长342 nm。结果:UV法研究表明萘酚喹在5.1～15.3 mg.L-1线性关系良好(r=0.999 9),平均回收率为100.0%,RSD 0.3%(n=9);HPLC法研究表明萘酚喹在0.16～0.8μg与峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.9%,RSD 0.2%(n=9)。UV,HPLC与非水溶液滴定法对比测定结果无明显差异。结论:UV与HPLC均可用于萘酚喹的含量测定。
OBJECTIVE: To compare the determination of naphthoquine by ultraviolet-visible spectrophotometry and high performance liquid chromatography (HPLC), and to provide basis for the establishment of quantitative method of naphthoquine. Methods: The sample was dissolved in 0.05 mol.L-1 hydrochloric acid by UV method at 341 nm. The HPLC column was Diamonsil C18 (4.6 mm × 250 mm, 5 μm). The mobile phase was acetonitrile-0.25% diethylamine ) (23:77), flow rate 0.8 mL.min-1, detection wavelength 342 nm. Results: The results showed that the naphthoquine had a good linearity (r = 0.999 9) at a linear range of 5.1-15.3 mg · L-1 with an average recovery of 100.0% and a RSD of 0.3% (n = 9) The linear range was 0.16-0.8 μg (r = 0.999 9) for quinoline with an average recovery of 99.9% and RSD 0.2% (n = 9). UV, HPLC and non-aqueous titration contrast test results no significant difference. Conclusion: UV and HPLC can be used for the determination of naphthoquine.