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目的 :拟在白噪声下观察对大鼠学习记忆的影响及海马区NOS的变化 ,探讨噪声所致大鼠学习记忆下降的机制。方法 :44只SD大鼠 ,其中 2 0只随机分两组 ,分别在持续 80dB (A)白噪声和无噪声的环境下 ,在Y -迷宫中进行空间辨别学习记忆训练 ,了解噪声对大鼠学习记忆的影响 ,另 2 4只大鼠随机分三组 :噪声训练组、正常训练组和噪声观察组 ,以同样的方法训练 ,用免疫组化的方法观察持续噪声对海马区NOS阳性神经元表达变化。结果 :噪声能降低大鼠学习记忆能力 ,而且海马区NOS阳性神经元较正常对照组的数量及染色强度显著降低。结论 :噪声降低海马区神经元活性 ,NOS的合成减少 ,抑制海马习得性长时程突触增强 ,影响记忆的获得与保持 ,延迟短时记忆向长时记忆的转化
Objective: To observe the effect of white noise on learning and memory of rats and the changes of NOS in hippocampus, and to explore the mechanism of noise-induced decline of learning and memory in rats. Methods: Forty - four SD rats were randomly divided into two groups. Sprague - Dawley (SD) rats were randomly divided into two groups, and trained in Y - maze with white noise and no noise. Learning and memory. The other 24 rats were randomly divided into three groups: noise training group, normal training group and noise observation group. Training in the same way, the effect of continuous noise on NOS positive neurons in hippocampus was observed by immunohistochemistry Change of expression Results: Noise reduced the ability of learning and memory in rats, and the number and staining intensity of NOS positive neurons in hippocampus were significantly lower than those in normal controls. CONCLUSION: Noise reduces the activity of neurons in the hippocampus, decreases the synthesis of NOS, inhibits the long-term hippocampal synaptic enhancement, affects the acquisition and maintenance of memory, delays the conversion of short-term memory to long-term memory