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目的分析新疆哈萨克族人群高血压与血浆免疫球蛋白G(Ig G)N-糖基组的关联,寻找高血压疾病状态的潜在分子标志物。方法采用简单随机抽样,选取2014年2—6月于新疆伊犁哈萨克自治州琼博拉乡卫生院建立健康档案的哈萨克族居民92例为研究对象,根据高血压诊断标准,将研究对象分为血压正常组(n=35)、正常高值组(n=27)和高血压组(n=30)。采用液相色谱-电喷雾质谱联用技术检测血浆Ig G N-糖基组。结果每例受试者的各Ig G亚型均检测到G0、G0F、G0FN、G0N、G1、G1F、G1FN、G1FNS、G1FS、G1N、G1NS、G1S、G2、G2F、G2FN、G2FNS、G2FS、G2N、G2NS、G2S等20种聚糖结构。除G0外,不同Ig G亚型各N-聚糖水平比较,差异均有统计学意义(P<0.05)。高血压组Ig G1亚型G0F、G0FN、G1FS水平高于血压正常组,G2、G2FS水平低于血压正常组;正常高值组Ig G1亚型G2F水平低于血压正常组,高血压组Ig G1亚型G2F水平低于血压正常组、正常高值组(P<0.05)。正常高值组Ig G2/3亚型G0FN、G0N水平高于血压正常组,G2、G2S水平低于血压正常组;高血压组Ig G2/3亚型G0、G0F、G0FN、G0N水平高于血压正常组,G2、G2F、G2FS、G2NS、G2S水平低于血压正常组,G2FN、G2FNS水平低于血压正常组、正常高值组(P<0.05)。高血压组Ig G4亚型G0FN水平高于血压正常组、正常高值组(P<0.05)。正常高值组Ig G1亚型半乳糖基化水平低于血压正常组,高血压组Ig G1亚型半乳糖基化水平低于血压正常组和正常高值组(P<0.05)。正常高值组、高血压组Ig G2/3亚型半乳糖基化、唾液酸化水平低于血压正常组,高血压组Ig G2/3亚型半乳糖基化水平低于正常高值组(P<0.05)。结论新疆哈萨克族人群不同血压状态与其血浆Ig G N-糖基组谱型变化相关,Ig G1亚型G2F水平及Ig G1、Ig G2/3亚型半乳糖基化水平的改变可能是该人群高血压疾病状态潜在的早期预警分子标志物和治疗新靶点。
Objective To analyze the association between hypertension and plasma IgG N-glycosyl group in Kazak ethnic population in Xinjiang and to search for potential molecular markers for hypertension. Methods Using simple random sampling, 92 Kazakh residents who established health records in Qionglaola Township Health Center, Yili Kazakh Autonomous Prefecture, Xinjiang Autonomous Region from February to June of 2014 were selected as study subjects. According to the diagnostic criteria of hypertension, the subjects were divided into normal blood pressure Group (n = 35), normal high value group (n = 27) and hypertension group (n = 30). Plasma Ig G N-glycosylation was detected by liquid chromatography-electrospray ionization mass spectrometry. Results G0, G0F, G0FN, G0N, G1, G1F, G1FN, G1FNS, G1FS, G1N, G1NS, G1S, G2, G2F, G2FN, G2FNS, G2FS, G2N were detected for each IgG subtype of each subject , G2NS, G2S and other 20 kinds of glycan structures. Except for G0, there were significant differences in the levels of N-glycans in different Ig G subtypes (P <0.05). The level of G0F, G0FN and G1FS in hypertensive group was higher than that in normal blood group, the level of G2 and G2FS was lower than that in normal blood pressure group. The level of IgF subtype G2F in normal high value group was lower than that in normal blood pressure group and hypertensive group Subgroup G2F levels were lower than those in normotensive and normal high-value groups (P <0.05). The levels of G0FN and G0N in Ig G2 / 3 subtypes in normal high-value group were higher than those in normal blood pressure group, while the levels of G2 and G2S were lower than those in normal blood pressure group. The levels of G0, G0F, G0FN and G0N in Ig G2 / The levels of G2, G2F, G2FS, G2NS and G2S in the normal group were lower than those in the normotensive group, while the levels of G2FN and G2FNS in the normotensive group were lower than those in the normotensive group and the normal high value group (P <0.05). The G0FN level of Ig G4 subtype in hypertensive group was higher than that in normal blood pressure group and normal high value group (P <0.05). Ig G1 subtype galactosylation in normal high value group was lower than that in normal blood pressure group, while Ig G1 subtype galactosylation level in hypertension group was lower than normal blood pressure group and normal high value group (P <0.05). The levels of galactosylated and sialylated Ig G2 / 3 subtype in hypertensive group were lower than those in normotensive group, and the level of Ig G2 / 3 subtype galactosylated in hypertensive group was lower than that in normal high group <0.05). Conclusion The different blood pressure status of Kazak people in Xinjiang is related to the change of IgG N-glycosylation profiles in plasma. The changes of IgF subtypes G2F and the levels of Ig G1 and Ig G2 / 3 subunits galactosylation may be the high Potential early warning molecular markers of blood pressure conditions and new targets for treatment.