论文部分内容阅读
目的观察异丙酚对培养海马神经元缺氧复氧后损伤反应的影响。方法培养12d海马神经元,随机分为正常对照再培养24h组(Nor组)、缺氧4h后复氧24h组(Ano组)、加异丙酚500μmol/L缺氧4h后复氧24h组(Pro组)。采用MTT法测定细胞存活率,免疫组化方法测定nNOS蛋白含量,应用流式细胞仪测定神经元凋亡率。结果缺氧后Ano组nNOS蛋白表达及细胞凋亡率明显增加(P﹤0.01),加入异丙酚后能减少nNOS蛋白含量(P﹤0.01)和细胞凋亡率(P﹤0.05),Pro组细胞存活率较Ano组增加(P﹤0.05),但与Nor组比仍下降(P﹤0.05)。结论异丙酚能提高体外海马神经元的抗缺氧能力,减少nNOS蛋白过度表达和细胞的凋亡。
Objective To investigate the effect of propofol on the injury response of cultured hippocampal neurons to hypoxia-reoxygenation. Methods The hippocampal neurons were cultured on the 12th day. They were randomly divided into two groups: control group (Nor group), normal group (Nor group), hypoxia 4h group (Ano group), propofol 500μmol / L group Pro group). The cell viability was determined by MTT assay. The content of nNOS protein was determined by immunohistochemistry. The apoptosis rate of neurons was determined by flow cytometry. Results After hypoxia, the expression of nNOS protein and the apoptosis rate of Ano group were significantly increased (P <0.01). After adding propofol, the content of nNOS protein and apoptosis rate were decreased (P <0.01) and Pro group The cell survival rate was higher than that of Ano group (P <0.05), but still lower than that of Nor group (P <0.05). Conclusion Propofol can increase hippocampal neurons against hypoxia in vitro and reduce the overexpression of nNOS and cell apoptosis.