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目的建立稳定表达荧光素酶的人乳腺癌细胞株并构建适用于小动物活体成像系统观察的裸鼠皮下移植瘤模型。方法采用脂质体将携带荧光素酶基因的质粒转染到人乳腺癌细胞株MCF-7中,G418筛选出稳定表达荧光素酶的单克隆细胞株。扩增后接种于裸鼠,建立裸鼠皮下移植瘤模型,通过活体动物成像系统监测肿瘤的生长过程。结果获得了高水平稳定表达荧光素酶的乳腺癌单克隆细胞株,该单克隆细胞株与母细胞系MCF-7具有相似的生长特性。将稳定表达荧光素酶的克隆接种于裸鼠皮下可成瘤,小动物活体成像系统能准确监测肿瘤细胞在体内的生长过程。结论成功建立了稳定表达荧光素酶的乳腺癌单克隆细胞株。采用活体动物成像系统构建的裸鼠皮下移植瘤模型是拓展肿瘤体内生长、转移及治疗相关研究的理想模型。
OBJECTIVE: To establish a human breast cancer cell line stably expressing luciferase and to construct a subcutaneous xenograft model in nude mice which is suitable for the observation of live animal imaging system. Methods The plasmid carrying luciferase gene was transfected into human breast cancer cell line MCF-7 by liposome. The monoclonal cell line stably expressing luciferase was screened by G418. After inoculation in nude mice, a subcutaneous xenograft tumor model was established in nude mice. The growth of the tumor was monitored by live animal imaging system. The results obtained a high level of stable expression of luciferase monoclonal antibody monoclonal cell lines, the monoclonal cell line and the mother cell line MCF-7 has similar growth characteristics. Inoculation of luciferase-expressing clones into the subcutaneous tumor of nude mice, small animal live imaging system can accurately monitor the growth of tumor cells in vivo. Conclusion A monoclonal cell line stably expressing luciferase was established successfully. The subcutaneous xenograft model in nude mice constructed by living animal imaging system is an ideal model to expand the research on the growth, metastasis and therapy of tumors in vivo.