目的研究大鼠晶状体海水浸泡伤后Na+,K+浓度和Na+,K+-ATP酶在mRNA水平的表达与形态学损伤之间的关系。方法用逆转录聚合酶链式反应法测定大鼠晶状体Na+,K+-ATP酶α亚单位在mRNA水平的表达状况,并与Na+,K+浓度和超微结构的变化进行对比分析。结果海水浸泡大鼠晶状体Na+,K+-ATP酶的α1,α2,α3亚单位mRNA水平的表达首先代偿性增高,随后呈失代偿性降低,并随时间的延长而逐渐降低。海水浸泡30 m in缝合伤口观察1周组的α1及α2亚单位的表达高于对照组,α3亚单位的表达基本恢复正常。结论在晶状体海水浸泡伤的病变过程中,Na+,K+-ATP酶亚单位活性的表达有不同变化。 Objective To investigate the relationship between Na +, K + concentration and Na +, K + -ATPase mRNA expression and morphological damage after lens seawater immersion injury in rats. Methods The mRNA expression of Na +, K + -ATPase α subunits in rat lens was determined by reverse transcription-polymerase chain reaction (RT-PCR). The changes of Na +, K + concentration and ultrastructure were compared. Results The mRNA expression of α1, α2 and α3 subunits of Na + and K + -ATPase in the seawater soaked rats firstly increased with compensatory increase and then decreased with decompensation, and then gradually decreased with the prolongation of time. The expression of α1 and α2 subunits in seawater immersion 30 m in suture wounds for 1 week group was higher than that in control group, and the expression of α3 subunit returned to normal. Conclusions The expression of Na +, K + -ATPase subunits in lens seawater immersion lesion varies with time.