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目的:探讨NF-κB在外周耐受发生中的活性改变及其意义。方法:以BALB/c小鼠为供者,输注1×108个供者脾细胞3d和7d的C3H小鼠为受者,建立皮肤移植模型。每天观察移植物的生长情况并于术后第7天取移植皮片和脾脏,通过对移植物的存活时间、组织病理学检查和抗原特异性淋巴细胞增殖试验来了解免疫功能的改变;以免疫组化染色法检测小鼠脾脏和皮肤移植物中NF-κBp65活性的变化。结果:供者特异性脾细胞输注后第7天进行手术,可使皮肤移植物的存活时间延长、抗原特异性淋巴细胞增殖反应明显受抑制;皮肤移植物中NF-κBp65的活性增加,脾脏NF-κBp65的活性降低。结论:输注供者特异性脾细胞诱导的外周耐受,可能与不同部位的免疫细胞内NF-κBp65活性改变有关。
Objective: To investigate the change of NF-κB activity in peripheral resistance and its significance. Methods: BALB / c mice were used as donors, and 3 × 7 days and 1 × 108 donor splenocytes were transfused into C3H mice as recipients to establish a skin graft model. The growth of the grafts was observed every day and the grafts and spleens were taken on the 7th day after surgery. The survival time of the grafts, histopathological examination and antigen-specific lymphocyte proliferation test were used to understand the changes of immune function. The changes of NF-κBp65 activity in mouse spleen and skin grafts were detected by histochemical staining. Results: The donor-specific splenocytes were operated on the 7th day after transfusion, prolonging the survival time of the skin grafts and inhibiting the proliferation of antigen-specific lymphocytes. The activity of NF-κBp65 in the skin grafts increased, NF-κBp65 activity decreased. CONCLUSION: Peripheral tolerance induced by donor-specific donor splenocytes may be related to the alteration of NF-κB p65 activity in different parts of immune cells.