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目的了解2014年深圳市龙岗区诺如病毒胃肠炎暴发疫情的分子流行病学特征。方法收集监测期间病毒性胃肠炎暴发疫情患者的肛拭子样本,采用实时荧光定量RT-PCR方法检测诺如病毒核酸,并选择部分阳性样本扩增VP1基因区,阳性产物回收并测序分析。结果 13起暴发疫情共检测标本157份,诺如病毒GⅡ型阳性25份,阳性率为15.9%,未检出诺如病毒GⅠ型。测序获得来自7起疫情的GⅡ型诺如病毒VP1基因序列,5株为GⅡ.4亚型,为新型变异株GII.4_Sydney 2012,2株GⅡ.17亚型,与2014年深圳本地株(KP718662)亲缘关系最近。结论 2014年深圳市龙岗区诺如病毒腹泻暴发的主要型别为GⅡ,并以GⅡ.4为主,流行优势株为GII.4_Sydney 2012。
Objective To understand the molecular epidemiological characteristics of Norovirus gastroenteritis outbreak in Longgang District of Shenzhen City in 2014. Methods The samples of anal swab in patients with outbreak of viral gastroenteritis during the surveillance period were collected. The real-time fluorescent quantitative RT-PCR method was used to detect the Norovirus nucleic acid. The positive samples were selected to amplify the VP1 gene. The positive products were recovered and sequenced. Results 157 outbreaks were detected in 13 outbreaks, of which 25 were Norovirus positive. The positive rate was 15.9%. No norovirus GⅠ was detected. The sequences of VP1 gene of GⅡnoviruses from seven outbreaks were sequenced and five of them were GⅡ.4 subtypes. The new variant strains GII.4_Sydney 2012 and the two GⅡ.17 subtypes were sequenced and compared with the native strains of Shenzhen in 2014 (KP718662 ) Kinship recently. Conclusion The main genotype of Norovirus virus diarrhea in Longgang District in 2014 was GⅡ, with GⅡ.4 as the predominant strain and GII.4_Sydney 2012 as the predominant strain.