During the non-landing measuring of vehicle mounted theodolite, especially under high-speed tracking measurement, the misalignment of theodolite’s center of mass and spindle etc. will cause high-frequency vibration of theodolite platform, increase the observation error of targets and even unbelievable results. In this paper, a correction method of non-landing measuring of theodolite based on static datum conversion is presented, which can effectively improve the observation accuracy of theodolite. The CCD camera is fixed to the theodolite platform to calculate the gesture shaking quantity of theodolite platform in geodetic coordinate system through the real time imaging of static datum. The observation results of theodolite are corrected by using such shaking quantity. The experiment shows that the correction accuracy exceeds 10 s of arc. The intrinsic parameter calibration technology of camera based on stellar angular distance and absolute conic put forward in this paper can prevent the estimated error of extrinsic parameters influencing the intrinsic parameter calibration and improve the intrinsic parameter calibration accuracy; the static datum conversion technology can reduce the influence of installation error of camera and theodolite platform on gesture measuring of the platform. The simulation experiment shows that when the shaking range of the platform is less than 30 min of arc, the influence of the three-axis installation error of camera within 3deg on the accuracy of correction results is less than 8 s of arc. The method in this paper can be extended to and used in the field of gesture shaking measuring and micro-structure deformation of various unstable platforms, therefore it is of important theoretical research significance and has wide engineering application prospect. During the non-landing measuring of vehicle mounted theodolite, especially under high-speed tracking measurement, the misalignment of theodolite’s center of mass and spindle etc. will cause high-frequency vibration of theodolite platform, increase the observation error of targets and even unbelievable results . In this paper, a correction method of non-landing measuring of theodolite based on static datum conversion is presented, which can effectively improve the observation accuracy of theodolite. The CCD camera is fixed to theodolite platform to calculate the gesture shaking quantity of theodolite platform in geodetic coordinate system through the real time imaging of static datum. The observation results of ofodolite are corrected by using such shaking quantity. The experiment shows that the correction accuracy exceeds 10 seconds of arc. The intrinsic parameter calibration technology of camera based on stellar angular distance and absolute conic put forward in this paper can prevent t he estimated error of extrinsic parameters influencing the intrinsic parameter calibration and improve the intrinsic parameter calibration accuracy; the static datum conversion technology can reduce the influence of installation error of camera and theodolite platform on gesture measuring of the platform. The simulation experiment shows that when the shaking range of the platform is less than 30 min of arc, the influence of the three-axis installation error of camera within 3 deg on the accuracy of correction results is less than 8 s of arc. The method in this paper can be extended to and used in the field of gesture shaking measuring and micro-structure deformation of various unstable platforms, therefore it is of important important theoretical research significance and has wide engineering application prospect.