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目的:观察和胃降逆方对非糜烂性胃食管反流病(NERD)大鼠5-羟色胺(5-hydroxy tryptamine,5-HT)及其受体表达的影响,探讨和胃降逆方对非糜烂性胃食管反流病大鼠的作用机制。方法:将SPF级SD大鼠随机分为空白组、模型组、奥美拉唑组、和胃降逆方高剂量组、和胃降逆方中剂量组、和胃降逆方低剂量组,采用基础致敏联合酸灌注的方法建立内脏高敏感NERD模型;干预2周后取血清与食管组织;ELISA法测定大鼠血清及食管中5-HT的含量,HE染色评价大鼠食管病理改变情况,免疫组织化学法测定5-HT3R与5-HT4R的表达水平。结果:和胃降逆方中、低剂量组、奥美拉唑组大鼠血清5-HT含量均低于模型组(P<0.05);和胃降逆方中剂量组、奥美拉唑组大鼠食管5-HT含量低于模型组(P<0.05);和胃降逆方高、中剂量组大鼠食管5-HT3R表达量明显低于模型组(P<0.05)。结论:和胃降逆方可降低NERD大鼠血清及食管5-HT水平,其作用机制可能与降低食管5-HT3R的表达有关。
Objective: To observe the effect of Hewei Jiangong on the expression of 5-hydroxytryptamine (5-HT) and its receptor in rats with non-erosive gastroesophageal reflux disease (NERD), and to explore the relationship between stomach reflux and inverse The role of non-erosive gastroesophageal reflux disease in rats. METHODS: Sprague-Dawley rats were randomly divided into control group, model group, omeprazole group, high-dose group of Weijiang Congfang group, middle-dose group of Weijiang inverse decoction group, and low-dose group of Weijiang inverse group. The visceral hypersensitivity NERD model was established by basic sensitization combined with acid perfusion; serum and esophageal tissue were taken after 2 weeks; serum and esophageal 5-HT levels were determined by ELISA; and esophageal pathological changes were evaluated by HE staining. The expression levels of 5-HT3R and 5-HT4R were determined by immunohistochemistry. RESULTS: The serum 5-HT levels in the rats in the middle and low dose groups and omeprazole group were lower than those in the model group (P<0.05); and the Weijiang inverse middle dose group and omeprazole group The content of 5-HT in the esophagus of the rats was lower than that in the model group (P<0.05). The expression of 5-HT3R in the esophagus in the high- and middle-dose group was significantly lower than that in the model group (P<0.05). Conclusion: Heweijiangni can reduce the serum and esophageal 5-HT levels in NERD rats, and its mechanism may be related to the decrease of esophageal 5-HT3R expression.