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应用改进的斑点免疫技术在国产微孔滤膜上检测柑桔溃疡病菌（Ｘａｎ－ｔｈｏｍｏｎａｓａｘｏｎｏｐｏｄｉｓｐｖ．ｃｉｔｒｉ）。结果表明，靶细菌检测下限为１×１０４细胞／ｍｌ，柑桔无症材料浸出液稀释（１２８倍）仍可检出。经柑桔叶面腐生黄单胞及近缘细菌交叉吸收，提高了多克隆抗血清特异性，能有效地避免假阳性。３种ＨＲＰ－标结结合物的效果为ＨＲＰ－ＳｐＡ＞ＨＲＰ－ＩｇＧ（鼠抗兔）＞ＨＲＰ－ＩｇＧ（羊抗兔）。封闭液采用ＴＢＳ＋０．１％曲拉通或０．５％吐温２０，吐温８０均可获得白色滤膜背景。对全国８省３８县５１８个柑桔无症样品ＤＩＡ检验结果，平均带菌率３１．８２％，符合实际病情。
The improved spot immunization technique was used to detect Citrus canker (Xan-thomonasaxonopodispv citri) on domestic microfiltration membrane. The results showed that the detection limit of target bacteria was 1 × 104 cells / ml, and the leaching of citrus asymptomatic material was still 128 times. The citrus leaf surface saprophytic xanthomonas and relative bacteria cross-absorption, increased polyclonal antiserum specificity, can effectively prevent false positives. The effect of the three HRP-labeled conjugates was HRP-SpA> HRP-IgG (mouse anti-rabbit)> HRP-IgG (goat anti-rabbit). The blocking solution was white background TBS + 0.1% Triton or 0.5% Tween 20, Tween 80. The results of DIA test on 518 citrus asymptomatic samples from 38 counties in 8 provinces in China show that the average carrying rate is 31.82%, which accords with the actual condition.