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目的:探讨人参皂苷Rg3对原代培养人喉鳞癌细胞中SIX1、TGF-β、VEGF-C表达的影响。方法:培养原代人喉鳞癌细胞,待细胞稳定传代后将其分为3组:A组(正常对照组)、B组(顺铂对照组)、C组(Rg3处理组)。Westernblot及细胞爬片免疫荧光检测各组细胞SIX1、TGF-β及VEGF-C蛋白的表达。RT-PCR检测各组细胞SIX1、TGF-β及VEGF-C mRNA的表达。结果:正常对照组细胞SIX1、TGF-β及VEGF-C蛋白及其mRNA表达均呈现强阳性。同正常对照组相比,顺铂对照组及Rg3处理组细胞TGF-β表达无明显变化,SIX1及VEGF-C蛋白及其mRNA表达下调,差异有统计学意义;同顺铂对照组相比,Rg3处理组细胞TGF-β表达无明显变化,SIX1及VEGF-C蛋白及mRNA表达出现了明显下调,差异有统计学意义。结论:Rg3能够通过降低人喉鳞癌细胞SIX1表达下调VEGF-C蛋白表达,进一步抑制喉鳞癌淋巴转移。同时提示,Rg3有比顺铂更强的抑制喉鳞癌淋巴转移的作用。
Objective: To investigate the effect of ginsenoside Rg3 on the expression of SIX1, TGF-β and VEGF-C in primary cultured human laryngeal squamous cell carcinoma. Methods: Primary human laryngeal squamous cell carcinoma cells were cultured and divided into 3 groups: A group (normal control group), B group (Cisplatin control group), C group (Rg3 treatment group). The expression of SIX1, TGF-β and VEGF-C in each group was detected by Western blot and cell slide immunofluorescence. The expression of SIX1, TGF-β and VEGF-C mRNA in each group was detected by RT-PCR. Results: SIX1, TGF-β and VEGF-C protein and mRNA expression in normal control cells showed strong positive. Compared with the normal control group, the expression of TGF-β in cisplatin control group and Rg3-treated group had no significant change, the expression of SIX1 and VEGF-C protein and its mRNA were down-regulated, the difference was statistically significant; compared with cisplatin control group, There was no significant change in the expression of TGF-β in Rg3-treated cells, and the expression of SIX1 and VEGF-C protein and mRNA were significantly down-regulated. The difference was statistically significant. Conclusion: Rg3 can inhibit the expression of SIX1 in human laryngeal squamous cell carcinoma cells by down-regulating the expression of VEGF-C protein and further inhibiting the lymph node metastasis of laryngeal squamous cell carcinoma. At the same time, Rg3 has stronger inhibitory effect on lymphatic metastasis of laryngeal squamous cell carcinoma than cisplatin.