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目的:分析河南西南部高危HPV基因型的分布及年龄特征,寻找优势基因型。方法:临床常规收集具有宫颈病变的宫颈脱落细胞,DNA抽提,荧光定量PCR进行分析,数据输入SPSS11.5,分析基因分布特征,确定优势基因型。结果:检测宫颈脱落细胞共468份,高危HPV检出127份,阳性率为27.1%。高危HPV基因型中,排列前5位的基因型依次为58(24.87%)、16(16.58%)、56(12.59%)、52(8.80%)、18(8.29%),其中58型感染率最高,为优势基因型。感染类型以混合型为主,占69.58%。高危HPV感染率最高在34岁以下,差异具有统计学意义(P<0.05)。结论:河南西南部高危HPV感染以58型为优势基因,有其地域特征,为该地区在高危HPV感染的预防指导、病毒基因型检测及疫苗设计的进行奠定基础。
Objective: To analyze the distribution and age characteristics of high-risk HPV genotypes in southwestern Henan and find the dominant genotypes. Methods: The cervical exfoliated cells with cervical lesions were collected routinely. DNA extraction and fluorescence quantitative PCR were used to analyze the data. The data were input into SPSS11.5 to analyze the gene distribution characteristics and determine the dominant genotypes. Results: A total of 468 cervical exfoliated cells were detected, and 127 high-risk HPVs were detected, the positive rate was 27.1%. Among the high risk HPV genotypes, the top five genotypes were 58 (24.87%), 16 (16.58%), 56 (12.59%), 52 (8.80%) and 18 Highest, the dominant genotype. The main types of infection were mixed type, accounting for 69.58%. The highest risk of high-risk HPV infection in 34 years of age, the difference was statistically significant (P <0.05). CONCLUSION: The high risk HPV infection in southwestern Henan province is characterized by its type 58 with its geographical features, which lays the foundation for the prevention and control of high-risk HPV infection, detection of virus genotypes and vaccine design in this area.