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目的:研究化浊解毒方对胃癌细胞血管内皮生长因子(VEGF)及环氧合酶-2(COX-2)表达的调控作用。方法:以不同剂量的化浊解毒方给Wistar大鼠灌胃制备含药血清,然后以含药血清培养胃癌细胞,共同培养2代后,分别用免疫组化法检测VEGF、COX-2的表达情况。结果:化浊解毒方高、中、低剂量组胃癌细胞中VEGF、COX-2的表达减弱,与对照组比较,差异均有显著性意义(P<0.01);而且高剂量组表达减弱更为明显,与低、中剂量组与较,差异均有非常显著性意义(P<0.01)。结论:化浊解毒方对胃癌细胞VEGF及COX-2的表达有抑制作用。
Objective: To study the regulation effect of Huazhuo Jiedu Decoction on the expression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) in gastric cancer cells. Methods: Different doses of Huazhuo Jiedu Decoction were used to prepare gavage serum of Wistar rats, and then the gastric cancer cells were cultured with the drug-containing serum. Co-cultured for 2 generations, the expressions of VEGF and COX-2 were detected by immunohistochemistry Happening. Results: The expression of VEGF and COX-2 in Huazhuo Jiedu Decoction high, medium and low dose groups were significantly lower than that in the control group (P <0.01), and the expression of VEGF and COX-2 in the high dose group was weaker Significantly, and low and middle dose groups and compared, the difference was significant (P <0.01). Conclusion: Huazhuo Jiedu Decoction can inhibit the expression of VEGF and COX-2 in gastric cancer cells.