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目的:研究再生障碍性贫血(AA)患者患病过程中血清IL-2、IL-8、TNF-α、IFN-γ和外周血T淋巴细胞亚群变化与疾病严重程度相关性。方法:选取我院经酶联免疫吸附法(ELISA)筛选的AA患者40例,其中急性患者(AAA)20例,慢性患者(CAA)20例,随机抽取20例健康人,对比监测60例目标病例的血清IL-2、IL-8、TNF-α、IFN-γ和外周血T淋巴细胞亚群变化。结果:AA组患者中AAA的各项细胞因子CD3+、CD4+、CD4+/CD8+检测结果较CAA患者波动较大。AA患者的血清IL-2、IL-8、TNF-α、IFN-γ含量明显高于健康人员平均值。AA患者的CD4+、CD4+/CD8+低于含量明显低于健康人员平均值,CD3+变化差异无统计学意义,AA组CD8+显著高于对照组,差异有统计学意义,P<0.05。结论:再障患者发病过程中同时存在细胞免疫功能障碍和体液免疫机制异常两种情况,且变化规律与疾病轻重缓急程度正相关。
Objective: To study the correlation between the changes of serum IL-2, IL-8, TNF-α, IFN-γ and peripheral blood T lymphocyte subsets and severity of disease in the patients with aplastic anemia (AA). Methods: Forty patients with AA were screened by enzyme-linked immunosorbent assay (ELISA) in our hospital, including 20 acute patients (AAA) and 20 chronic patients (CAA). 20 healthy subjects were randomly selected and compared with 60 patients Cases of serum IL-2, IL-8, TNF-α, IFN-γ and peripheral blood T lymphocyte subsets changes. Results: The results of AAA, CD3 +, CD4 +, CD4 + / CD8 + in patients with AA were higher than those in CAA patients. The serum levels of IL-2, IL-8, TNF-α and IFN-γ in AA patients were significantly higher than those of healthy individuals. The levels of CD4 + and CD4 + / CD8 + in patients with AA were significantly lower than those in healthy individuals, but there was no significant difference in CD3 + between the two groups. The level of CD8 + in AA group was significantly higher than that in control group (P <0.05). CONCLUSION: There are two cases of cellular immune dysfunction and abnormal humoral immune function in the pathogenesis of aplastic anemia. The regularity of the changes is positively correlated with the severity of the disease.