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据报道,硫酸粘多糖分子(聚阴离子)和碱性染料(阳离子)间相互作用引起的异染性(metachromasia)可能是指示这类高分子改变它的受体(如蛋白质)性质的能力的一种简便检测的模型。刺参酸性粘多糖(下称 SJAMP)是一种无脊椎动物硫酸粘多糖。为了对比 SJAMP 和已知的哺乳动物硫酸粘多糖异染活性的异同,我们观察了 SJAMP、肝素(下称 Hep)、硫酸软骨素(下称 CSA)与碱性染料天青Ⅰ相互作用所致的吸收光谱的变化。我们采用日立330型分光
It has been reported that the metachromasia caused by the interaction between the mucopolysaccharide molecules (polyanions) and basic dyes (cations) may be one that indicates the ability of such polymers to alter the properties of their receptors (eg, proteins) A simple model of detection. Acanthopanax senticosus acid mucopolysaccharide (SJAMP) is an invertebrate mucopolysaccharide. In order to compare the similarities and differences between SJAMP and the known mammalian mucoitin heterosomal activity, we observed the interaction between SJAMP, heparin (Hep), chondroitin sulfate (hereinafter CSA) and basic dye Azure I Absorption spectrum changes. We use Hitachi 330 spectroscopy