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[目的]考查培养基中铜元素和锌元素的含量对小麦胚芽愈伤组织维生素E积累的影响。[方法]通过建立维生素E积累和细胞生长的数学模型,描述小麦胚芽愈伤组织的维生素E积累和细胞增长。以次生代谢产物维生素E积累最多为目标,通过试验优化确定铜锌含量的最佳组合。[结果]结果表明,当B5培养基中ZnSO4.7H2O为2.0mg/ml;CuSO4.5H2O为0.1mg/ml时,有利于维生素E积累。维生素E积累的动力学模型的拟合度为97.53%,细胞干重的动力学模型拟合度为95.60%,非线性关系良好。[结论]微量元素铜和锌对小麦胚芽愈伤组织维生素E积累有影响,铜元素较锌元素的影响显著,且低浓度的铜锌之间有协同作用;随着浓度增大,抑制作用逐渐增强。
[Objective] The research aimed to investigate the effects of copper and zinc on the accumulation of vitamin E in wheat germ callus. [Method] The mathematical model of vitamin E accumulation and cell growth was established to describe the vitamin E accumulation and cell growth of wheat germ callus. To secondary metabolites of vitamin E accumulation of up to the goal of optimizing the test to determine the optimal combination of copper and zinc content. [Result] The results showed that when ZnSO4.7H2O was 2.0mg / ml in B5 medium and 0.1mg / ml in CuSO4.5H2O, it was beneficial to vitamin E accumulation. The kinetic model of vitamin E accumulation was 97.53% and the kinetic model of cell dry weight was 95.60%. The nonlinearity was good. [Conclusion] The trace elements Cu and Zn had effects on the accumulation of vitamin E in callus of wheat germ. The effects of Cu and Zn were significant, and the synergistic effect was found between Cu and Zn at low concentration. With the increase of concentration, the inhibition was gradually Enhanced.