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目的:建立荆芥内酯的含量测定方法和有关物质的分析方法。方法:指定以Waters XTerra MS C18色谱柱进行有关物质分析,指定以Kromasil C18色谱柱进行含量测定,以甲醇-水(45∶55)为流动相(氢氧化钠调pH至10.0),流速为0.8mL·min-1,柱温30℃,检测波长216 nm。结果:荆芥内酯与有关物质及各降解成分完全分离;荆芥内酯质量浓度在0.012 6～0.403 6 g·L-1与峰面积呈良好线性关系,r=0.999 9;最低检测限0.25μg,最小定量限为0.63μg;平均回收率100.04%,RSD为1.40%。结论:该方法操作简便、快速、准确,灵敏度高,可用于荆芥内酯含量及有关物质的测定。
Objective: To establish a method for the determination of nepetalactone and its related substances. Methods: Specified Waters XTerra MS C18 column for the analysis of related substances, designated Kromasil C18 column with methanol - water (45:55) as the mobile phase (sodium hydroxide to pH 10.0) at a flow rate of 0.8mL · min-1, column temperature 30 ℃, detection wavelength 216 nm. Results: Nepetalactone was completely separated from the related substances and the degradation components. The linear range of the concentration of nepetalactone was 0.012 6 ~ 0.403 6 g · L-1 with the peak area, r = 0.999 9; the lowest detection limit was 0.25 μg, the minimum limit of quantification was 0.63μg; the average recovery was 100.04% with a RSD of 1.40%. Conclusion: The method is simple, rapid, accurate, sensitive and can be used for the determination of nepetalactone content and related substances.