论文部分内容阅读
目的研究doublecortin样激酶-1(DCLK1)在胃癌组织的表达情况及其与患者临床病理特征及预后的关系。方法收集北京协和医院2002年7月至2006年12月收治的122例胃癌根治患者的临床病理资料及标本,采用免疫组织化学法检测DCLK1在胃癌及癌旁组织中的表达水平,Kaplan-Meier法绘制生存曲线,Cox比例风险模型进行单因素及多因素生存分析。结果 122例胃癌患者中,51例存在DCLK1高表达。DCLK1与患者淋巴结转移(P=0.029)、脉管癌栓(P=0.029)密切相关。Kaplan-Meier分析结果显示,DCLK1高表达者的5年总生存率和5年无病生存率分别为39.0%和37.0%,明显低于DCLK1低表达者的65.8%和64.5%(P均=0.001)。Cox模型分析结果显示,DCLK1是胃癌患者总生存时间和无病生存时间的独立预后因素(P均=0.036)。结论 DCKL1高表达与胃癌淋巴结转移和脉管癌栓相关,是胃癌患者预后不良的一个潜在分子指标。
Objective To investigate the expression of doublecortin-like kinase-1 (DCLK1) in gastric cancer and its relationship with clinicopathological features and prognosis. Methods Clinicopathological data and specimens from 122 patients with radical gastrectomy who were treated in Peking Union Medical College Hospital from July 2002 to December 2006 were collected. The expression of DCLK1 in gastric cancer tissues and adjacent normal tissues was detected by immunohistochemistry. The Kaplan-Meier method The survival curves were drawn and the Cox proportional hazards model was used for univariate and multivariate survival analyzes. Results Among the 122 gastric cancer patients, DCLK1 was highly expressed in 51 cases. DCLK1 was closely related to lymph node metastasis (P = 0.029) and vascular tumor thrombus (P = 0.029). Kaplan-Meier analysis showed that the 5-year overall survival and 5-year disease-free survival of patients with high DCLK1 expression were 39.0% and 37.0%, respectively, which were significantly lower than 65.8% and 64.5% of those with low DCLK1 expression (P = 0.001) ). Cox model analysis showed that DCLK1 was an independent prognostic factor for overall survival and disease-free survival in patients with gastric cancer (P = 0.036). Conclusion The high expression of DCKL1 is associated with lymph node metastasis and vascular tumor thrombus in gastric cancer, which is a potential molecular marker of poor prognosis in patients with gastric cancer.