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目的 探讨HIV感染后机体NK(naturalkillercells)及NKT细胞的变化情况。方法 取外周血细胞 ,用标记荧光的抗体进行染色 ,流式细胞仪分析HIV AIDS患者NK和NKT细胞的变化。结果 HIV AIDS患者NK、NKT细胞和CD4 + T细胞显著低于正常对照 ;CD8+ T细胞显著高于正常对照。HIV AIDS患者NK百分数显著低于正常对照 ,与CD4 + T细胞数量成正比 ,r=0 .2 89,P <0 .0 1 ;NKT细胞数量与CD4 + T细胞数量成正比 ,r =0 .378,P <0 .0 1 ;与CD8+ T细胞数量成正比 ,r =0 .340 ,P <0 .0 1 ;长期不进展组NKT、NK细胞数量与正常对照组差异无显著性。结论 HIV感染可明显降低HIV AIDS患者NK和NKT细胞数量 ,NK和NKT细胞变化与疾病进展密切相关。
Objective To investigate the changes of natural killer cells and NKT cells after HIV infection. Methods Peripheral blood cells were obtained and stained with fluorescent antibody. The changes of NK and NKT cells in HIV AIDS patients were analyzed by flow cytometry. Results NK, NKT cells and CD4 + T cells in HIV AIDS patients were significantly lower than those in normal controls; CD8 + T cells were significantly higher than those in normal controls. NK percentage of HIV AIDS patients was significantly lower than the normal control, and the number of CD4 + T cells is proportional to, r = 0.298, P <0.01; NKT cells and CD4 + T cells is proportional to the number, r = 378, P <0.01; in proportion to the number of CD8 + T cells, r = 0.340, P <0.01; there was no significant difference between the number of NKT and NK cells in long-term progression group and normal control group. Conclusion HIV infection can significantly reduce the number of NK and NKT cells in HIV-infected patients. The changes of NK and NKT cells are closely related to the progression of the disease.