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目的 观察化学药物大鼠多发肾囊肿伴肾肿瘤模型的诱发情况和DNA损伤标记物 8 羟基脱氧鸟苷 (8 OHdG)在其肾组织的表达。 方法 用 2 氨基 4,5 二苯噻唑 (DPT)和N 亚硝基吗啉 (NNM)诱发与人类获得性肾囊肿 (ACDK)伴肾癌相似的大鼠动物模型。并用免疫组化方法 (LSAB法 )检测 8 OHdG在大鼠肾组织的表达。 结果 在本实验中 ,NNM组 3/ 10的大鼠诱发肾肿瘤出现。在DPT/NNM组的 9只大鼠均发生双侧肾脏多发囊性变化 ,7/ 9大鼠有肾肿瘤的发生 ,其中 4例肾肿瘤发生于囊肿上皮细胞并向囊腔内生长。用DPT/NNM诱发大鼠模型的病理改变与人类ACDK极为相似。在上述两实验组的大鼠肾脏组织中发现肾小管上皮、囊肿上皮、间质细胞及肿瘤细胞均有 8 O HdG的明显表达。 结论 用DPT和NNM能诱发与人类获得性肾囊肿 (ACDK)伴肾癌相似的大鼠动物模型 ,并能造成相似的病理改变和DNA损伤标记物 8 OHdG的明显表达 ,两者可能存在某些相似的发病机制。
Objective To observe the induction of multiple nephroureterectasis with renal tumor in chemical rats and the expression of DNA damage marker 8-OHdG in its kidney. Methods Rat models of human renal cell carcinoma resembling human acquired renal cysts (ACDK) were induced with 2-amino-4,5-diphenylthiazole (DPT) and nitrosomorpholine (NNM) The expression of 8-OHdG in rat kidney was detected by immunohistochemistry (LSAB). Results In this experiment, 3/10 rats in the NNM group developed renal tumors. In the DPT / NNM group, 9 rats had bilateral multiple cystic changes of the kidney, and 7 of 9 rats had renal tumors. Among them, 4 cases of renal tumors occurred in cyst epithelial cells and grew into cysts. Pathological changes in rat models induced by DPT / NNM are very similar to human ACDK. The expression of 8 O HdG in renal tubule epithelium, cyst epithelium, stromal cells and tumor cells was found in the rat kidney tissue of the two experimental groups. Conclusions Both DPT and NNM can induce rat models of human renal cell carcinoma (ACDK) associated with human acquired renal cysts (ACDK) and cause similar pathological changes and significant expression of DNA damaging marker 8 OHdG, both of which may have some Similar pathogenesis.