论文部分内容阅读
目的:检测食管鳞癌患者肿瘤组织及血清中E-钙黏蛋白(E-cadherin,E-cad)的表达并探讨其临床意义。方法:采用免疫组织化学法对48例食管鳞癌、23例糜烂性食管炎、24例正常食管黏膜组织中E-cad的表达进行检测;同时采用酶联免疫吸附法(ELISA)对上述研究对象血清中可溶性E-cad(sE-cad)的表达进行检测,并且收集完整的临床病理资料。结果:E-cad表达水平在食管鳞癌组织中明显降低,与正常食管黏膜上皮组织及糜烂性食管炎黏膜上皮组织相比,差异有统计学意义(P<0.05)。不同浸润深度、有无淋巴结转移及不同分化程度的食管鳞癌,其癌组织中E-cad的表达水平差异有统计学意义(P<0.05)。血清sE-cad在食管鳞癌组表达水平明显高于正常食管组及糜烂性食管炎组(P<0.05)。血清sE-cad水平与食管鳞癌的临床病理特征无关。食管鳞癌患者肿瘤组织中E-cad高表达者与低表达者相比,血清sE-cad水平差异无统计学意义(P=0.134)。结论:E-cad可作为食管鳞癌诊断及预后判断的辅助指标。血清sE-cad可作为食管鳞癌诊断筛查的血清学辅助指标。
Objective: To detect the expression of E-cadherin (E-cadherin) and its clinical significance in esophageal squamous cell carcinoma patients and its clinical significance. Methods: The expression of E-cadherin in 48 cases of esophageal squamous cell carcinoma, 23 cases of erosive esophagitis and 24 cases of normal esophageal mucosa were detected by immunohistochemical method. Meanwhile, the expression of E-cadherin in esophageal squamous cell carcinoma was detected by enzyme-linked immunosorbent assay (ELISA) Serum soluble E-cad (sE-cad) expression was detected, and the collection of complete clinical and pathological data. Results: The expression of E-cad in esophageal squamous cell carcinoma was significantly lower than that in normal esophageal mucosa epithelium and esophageal mucosa epithelium, the difference was statistically significant (P <0.05). The expression of E-cad in different depth of invasion, lymph node metastasis and differentiation of esophageal squamous cell carcinoma was significantly different (P <0.05). Serum sE-cad expression in esophageal squamous cell carcinoma was significantly higher than that in normal esophageal and erosive esophagitis groups (P <0.05). Serum sE-cad levels and esophageal squamous cell carcinoma of the clinicopathological features. There was no significant difference in serum sE-cad levels between esophageal squamous cell carcinoma patients with high expression of E-cadherin and low expression (P = 0.134). Conclusion: E-cad can be used as an auxiliary index for the diagnosis and prognosis of esophageal squamous cell carcinoma. Serum sE-cad can be used as a diagnostic serological marker of esophageal squamous cell carcinoma secondary indicators.