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目的探讨山莨菪碱对海水淹溺性肺水肿(PE-SWD)治疗作用。方法复制大鼠PE-SW模型后,将大鼠随机分为手术对照组(C组)、PE-SW模型组(M组)和山莨菪碱治疗组(T组)。采用全自动动脉血气分析仪测定各组大鼠动脉血气,使用酶联免疫吸附试验法(ELISA)测定个组大鼠血浆、支气管肺泡灌洗液(BALF)和肺组织中TNF-α含量,同时测定个组大鼠肺组织湿/干重变化。结果海水灌注后2h开始,T组大鼠PaO2、SO2、pH显著高于M组。肺组织湿/干重比值则低于M组;M和T组TNF-α在血浆、BALF和肺组织的浓度与对照组相比在淹溺后显著升高,1h到达峰值,后逐渐下降。海水灌注后,T组大鼠TNF-α均明显低于M组大鼠。结论山莨菪碱对海水淹溺性肺水肿有治疗作用,它的作用可能是通过对TNF-α的影响而产生的。
Objective To investigate the therapeutic effect of anisodamine on seawater drowning pulmonary edema (PE-SWD). Methods After the rat PE-SW model was duplicated, the rats were randomly divided into operation control group (C group), PE-SW model group (M group) and scopolamine treatment group (T group). Arterial blood gas was measured by automatic arterial blood gas analyzer, and the levels of TNF-α in plasma, bronchoalveolar lavage fluid (BALF) and lung tissue were measured by enzyme-linked immunosorbent assay (ELISA) The changes of wet / dry weight of lungs in each group were determined. Results After 2h of seawater infusion, PaO2, SO2 and pH of T group were significantly higher than those of M group. The wet / dry weight ratio of lung tissue was lower than that of M group. The concentrations of TNF-αin plasma, BALF and lung tissue in M and T groups were significantly increased after drowning, reaching the peak value at 1h and then gradually decreasing. After water instillation, TNF-α in T group was significantly lower than that in M group. Conclusions Anisodamine has a therapeutic effect on seawater flooding pulmonary edema, and its effect may be through the influence of TNF-α.