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对单芽茎段离体快繁获得的中国野生葡萄燕山-1(Vitis yeshanensis‘Yanshan~(-1)’)×河岸-3(V.riparia‘Hean-3’)杂种F_1代的31个株系、河岸-3和酿酒葡萄品种‘赤霞珠’(V.vinifera‘Cabernet Sauvignon’)的组培苗进行耐盐性筛选,以筛选出优良的耐盐株系作为砧木育种的材料。取离体快繁技术获得的试管苗中部生长较为一致的单芽茎段加入含有不同浓度NaCl的生根培养基中,培养40d内观察盐害症状,计算盐害指数,用相对耐盐指数对供试材料的耐盐性进行初步分级。通过筛选将供试的燕山-1×河岸-3杂种F_1代的31个株系分为5个类型:11个株系为高耐盐葡萄、5个株系为耐盐葡萄、7个株系为中耐盐葡萄、5个株系为盐敏感葡萄、3个为盐高敏葡萄。此外,河岸-3为高耐盐葡萄、‘赤霞珠’为盐敏感葡萄;燕山-1×河岸-3杂种F_1代的31个株系中有8个株系的耐盐表现优于父本河岸-3,出现超亲遗传现象。通过初步筛选,鉴定出11个高耐盐的杂种F_1代株系,可以作为优良的砧木材料。
Thirty-one strains of F_1 generation from the hybrids of Vitis yeshanensis’Yanshan ~ (-1) ’× V.riparia’Hean-3’ Line 3 and the wine grape variety V. vinifera Cabernet Sauvignon were screened for salt tolerance to screen out good salt tolerant lines as material for rootstock breeding. The single root shoots growing in the middle part of the vitro plantlets obtained from the in vitro rapid propagation technique were added to the rooting medium containing different concentrations of NaCl and the symptoms of salt damage were observed within 40 days after cultivation. The salt damage indices were calculated. The salt tolerance of the test material was initially graded. Thirty-one strains of F_1 generation of Yanshan-1 × riparian-3 hybrid F1 were divided into five types by screening: 11 were high salt-tolerant grapes, 5 were salt-tolerant grapes and 7 were For the salt-tolerant grapes, five strains were salt-sensitive grapes and three were salt-sensitive grapes. In addition, riparian-3 was high salt-tolerant grape and ’Cabernet Sauvignon’ was salt-sensitive grape. Among 31 lines of Yanshan-1 × riparian-3 hybrid F 1, 8 strains had better salt tolerance than male parent Riverbank -3, appeared super-genetic heredity. Through preliminary screening, 11 high salt tolerant hybrid F 1 lines were identified and could be used as excellent rootstocks.