论文部分内容阅读
目的采用血清群特异性PCR技术了解贵州省近年钩端螺旋体(钩体)流行菌群,为贵州省钩体病的防控提供技术手段和科学依据。方法采用致病性钩体特异性PCR方法(G1/G2-PCR)对来自贵州省近年的钩体分离菌株进行鉴定,进一步应用基于致病性钩体O抗原基因的血清群特异性PCR(O-PCR)对贵州省近年的58株钩体分离株进行血清群鉴定,并采用显微凝集试验(MAT)对O-PCR方法的检测结果进行验证。结果 G1/G2-PCR检测结果显示58株菌株均为致病性钩体,O-PCR将58株致病性钩体菌株鉴定为黄疸出血群,与传统的MAT法鉴定结果一致。结论 O-PCR技术可作为我省钩体快速分群鉴定可靠的实验室诊断技术手段,贵州省近年的钩体流行菌群为黄疸出血群。
Objective To understand the prevalence of Leptospira (Leptospira) in Guizhou Province in recent years by using serogroup-specific PCR technique and to provide technical and scientific evidence for the prevention and control of leptospirosis in Guizhou Province. Methods Isolates of Leptospira isolates from Guizhou province were identified by pathogen-specific leptospira-specific PCR (G1 / G2-PCR) and further serogroup-specific PCR based on the O antigen gene of pathogenic Leptospira (O -PCR) was used to identify the serogroups of 58 leptospira isolates in Guizhou province in recent years. The microaggregation test (MAT) was used to verify the results of O-PCR. Results The results of G1 / G2-PCR showed that 58 strains were all pathogenic leptospira, and 58 strains of pathogenic Leptospira were identified as hemorrhagic group by O-PCR, which was consistent with the traditional MAT method. Conclusion O-PCR technique can be used as a reliable laboratory diagnostic technique for quick cluster identification in our province. In recent years, the prevalence of hook-body bacteria in Guizhou Province is a hemorrhagic group of jaundice.