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用纤维素(Cellulose,CF-11)过滤去除白细胞后再用 Percoll 密度梯度离心方法提纯分离间日疟(P.v.)、恶性疟(P.f.)和伯氏疟(P.b.)3种疟原虫。经纤维素过滤后,P.v.病人血和P.b.感染鼠血中白细胞去除率分别平均达 94.7％和 75.4％,而且不影响原虫密度和各期构成比。多个 Percoll 不连续梯度离心测试3种疟原虫裂殖体、滋养体、环状体和未感染红细胞的相对平均密度(kg/L):P.v.分别为 1.059、1.072、1.096 和 1.099;P.f.分别为 1.070、1.079、1.10 和 1.10,配子体 1.085;P.b.分别为 1.059、1.062、1.103 和 1.104。并据此选择各自合适的 Percoll 梯度分离3 种疟原虫,将裂殖体、滋养体同环状体分开,获取原虫纯度(原虫数/100 个红细胞),P.v.由分离前 0.8％至分离后 93.5％,P.f.由2.3％至100％,P.b.由 30.6％至93％,分别提高 113.8、43.4和 2倍。
After the leukocytes were filtered off with Cellulose (CF-11) and purified by Percoll density gradient centrifugation, P. falciparum, P.f. and P.b. malariae were isolated. After cellulose filtration, the blood leukocyte removal rates in P.v. And P.b. infected mice averaged 94.7% and 75.4%, respectively, and did not affect protozoa density and stage composition ratio. The relative average density (kg / L) of three Plasmodium schizonts schizonts, trophozoites, rings and uninfected erythrocytes were measured by Percoll discontinuous gradient centrifugation: Pv were 1.059, 1.072, 1.096 and 1.099, respectively; Pf was 1.070, 1.079, 1.10 and 1.10, with gametophyte 1.085; Pb 1.059, 1.062, 1.103 and 1.104, respectively. According to this, we selected the appropriate Percoll gradient to separate the three species of Plasmodium, separated the schizonts and trophozoites from the annulus, and obtained the purity of the protozoa (protozoa / 100 erythrocytes). The Pv was from 0.8% before isolation to 93.5 %, Pf from 2.3% to 100%, Pb from 30.6% to 93%, respectively, increased 113.8,43.4 and 2 times.