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目的检测胃癌患者血清白细胞介素2(IL-2)和Fas配体(FasL)的表达,探讨其与胃癌临床及病理指标的关系。方法42例胃癌患者血清标本,其中男性26例,女性16例;年龄35～80岁,平均年龄60.0岁。同期正常对照者血清标本40例,其中男性25例,女性15例;年龄36～78岁,平均年龄58.8岁。应用酶联免疫吸附分析(ELISA)检测胃癌患者和正常对照者血清标本中IL-2和FasL的表达,作对比分析。同时采用单因素方差分析和Spearman相关分析探寻血清IL-2和FasL的表达与胃癌病理类型、病理分期及TNM分期的关系。统计分析采用SPSS16.0软件,P<0.05为差异有统计学意义。结果胃癌患者血清IL-2的表达显著低于正常对照者[(211.3±119.0)ng/mL vs(287.9±79.9)ng/mL;P=0.001];胃癌患者血清FasL的表达显著高于正常对照者[(2.7±1.0)ng/mL vs(1.9±1.2)ng/mL;P=0.001]。Spearman相关分析显示胃癌患者血清IL-2与FasL之间无显著相关关系。结论血清IL-2和FasL表达与胃癌的发病密切相关,可作为协同判断胃癌发病的指标。
Objective To detect the expression of interleukin 2 (IL-2) and Fas ligand (FasL) in patients with gastric cancer and to investigate their relationship with the clinical and pathological parameters of gastric cancer. Methods 42 cases of gastric cancer patients serum samples, including 26 males and 16 females; aged 35 to 80 years, mean age 60.0 years. The same period the normal control serum samples 40 cases, 25 males and 15 females; aged 36 to 78 years, mean age 58.8 years. The expression of IL-2 and FasL in serum samples from patients with gastric cancer and normal controls was detected by enzyme-linked immunosorbent assay (ELISA). At the same time, using single factor analysis of variance and Spearman correlation analysis to explore the relationship between serum IL-2 and FasL expression and gastric cancer pathological type, pathological stage and TNM staging. Statistical analysis using SPSS16.0 software, P <0.05 for the difference was statistically significant. Results The serum level of IL-2 in patients with gastric cancer was significantly lower than that in healthy controls [(211.3 ± 119.0) ng / mL vs (287.9 ± 79.9) ng / mL; P = 0.001] [(2.7 ± 1.0) ng / mL vs (1.9 ± 1.2) ng / mL; P = 0.001]. Spearman correlation analysis showed no significant correlation between serum IL-2 and FasL in gastric cancer patients. Conclusions The expression of IL-2 and FasL in serum is closely related to the onset of gastric cancer and can be used as an index to judge the incidence of gastric cancer.