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目的研究泽泻多糖对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用。方法分别取昆明小鼠60只,雌雄各半,随机分为6组,分别为正常组和模型组(灌胃给予蒸馏水),联苯双酯组(200 mg/kg),泽泻多糖低、中、高剂量组(300,600,1200 mg/kg),小鼠均ig给药,每天1次,连续7 d,末次给药2 h后,除正常组外,其他各组均ip CCl_4造成急性肝损伤。给药后测定小鼠肝脏系数和脾脏系数,测定CCl_4肝损伤小鼠血清中丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST),总胆红素(T-BIL),超氧化物歧化酶(SOD)活性,测定肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,苏木素-伊红(HE)染色观察肝损伤组织病理切片。结果与正常组比较,模型组CCl4肝损伤小鼠血清中AST,ALT,TBIL活性明显升高,而SOD活性明显降低(P<0.01);肝脏组织中SOD活性明显降低(P<0.01),MDA含量明显升高(P<0.01),模型组病理学观察肝损伤均较为明显。与模型组比较,泽泻多糖中、高剂量能够明显降低AST,ALT,T-BIL活性,同时明显升高SOD活性(P<0.05,P<0.01);并能明显够升高肝组织中SOD活性和明显降低MDA含量(P<0.05),病理检查结果显示泽泻多糖具有明显的保肝作用。结论泽泻多糖多小鼠CCl4肝损伤有良好的保护作用。
Objective To study the protective effect of Alisma orientale against acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods Sixty Kunming mice were randomly divided into 6 groups, which were divided into 6 groups: normal group and model group (intragastric administration of distilled water), bifendate group (200 mg / kg) Medium and high dose groups (300, 600, 1200 mg / kg), the mice were administered ig, once a day for 7 days. After the last administration for 2 hours, except for the normal group, ip CCl_4 caused acute liver in all other groups damage. After administration, the hepatic coefficient and spleen coefficient of mice were determined, and serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (T-BIL) , Superoxide dismutase (SOD) were measured. The activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue were determined. Hepatic tissue sections were observed by hematoxylin and eosin (HE) staining. Results Compared with the normal group, the activities of AST, ALT and TBIL in the serum of CCl4 liver injury model group were significantly increased, but the SOD activity was significantly decreased (P <0.01); the activity of SOD in liver tissue was significantly decreased (P <0.01) Content was significantly increased (P <0.01), the model group pathological observation of liver injury were more obvious. Compared with the model group, high and middle dose of Alisma orientalis polysaccharide could significantly decrease the activities of AST, ALT and T-BIL, and significantly increase the activity of SOD (P <0.05, P <0.01) Activity and significantly decreased MDA content (P <0.05). The results of pathological examination showed that Alisma orientalis polysaccharides had obvious hepatoprotective effects. Conclusion Alisma polysaccharides in mice CCl4 liver injury have a good protective effect.