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目的:建立坤复康胶囊中芍药苷的HPLC含量测定方法。方法:采用甲醇提取样品,C18色谱柱(4.6mm×150mm,5μm),流动相为乙腈-水(14∶86),检测波长230nm。结果:进样量在0.316~1.896μg范围内线性关系良好,r=0.99965,精密度、重复性、稳定性均符合相关规定,平均回收率97.57%,方法简便、灵敏、准确、重复性好,能有效测定坤复康胶囊中芍药苷的含量。结论:所建立的方法可用于坤复康胶囊中芍药苷含量测定。
Objective: To establish HPLC method for the determination of paeoniflorin in Kunfukang capsules. Methods: The samples were extracted with methanol, C18 column (4.6mm×150mm, 5μm). The mobile phase was acetonitrile-water (14∶86). The detection wavelength was 230nm. Results: The linear relationship was good in the range of 0.316~1.896μg, r=0.99965. The precision, repeatability and stability were all in accordance with relevant regulations. The average recovery rate was 97.57%. The method was simple, sensitive, accurate, and reproducible. It can effectively determine the content of paeoniflorin in Kun Fukang capsules. Conclusion: The established method can be used to determine the content of paeoniflorin in Kun Fukang capsules.