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目的了解和掌握明光市5岁以下住院儿童腹泻轮状病毒感染情况。方法收集明光市人民医院2012年5~11月5岁以下住院儿童腹泻粪便标本,采用酶联免疫法检测A组轮状病毒,阳性者反转录-聚合酶链反应进行G/P分型鉴定。结果 A组轮状病毒感染率35.33%,A组轮状病毒P分型以P[4]为主,G分型以G2为主。结论轮状病毒感染是明光市5岁以下住院儿童腹泻的重要病因。
Objective To understand and grasp the infection of rotavirus in children under 5 years of age in Mingguang City. Methods The specimens of diarrhea in hospitalized children under 5 years of age from May to November, 2012 in People ’s Hospital of Mingguang City were collected. The group A rotavirus was detected by enzyme - linked immunosorbent assay (ELISA), and the G / P typing was performed by reverse transcriptase - polymerase chain reaction . Results The infection rate of rotavirus in group A was 35.33%. The group A rotavirus P was predominantly P [4], and the group G was predominantly G2. Conclusion Rotavirus infection is an important cause of diarrhea in children under 5 years of age in Mingguang City.