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为了发掘新的抗赤霉病基因,以抗赤霉病新种质N553与扬麦13构建的包含184个家系的重组自交系(RILs)为材料,利用217对在双亲间具有多态性的分子标记构建遗传连锁图谱,利用该图谱对小穗密度、株高及赤霉病抗性进行QTL检测,并分析了小穗密度及株高与赤霉病抗性的相关性。结果表明,本研究共检测到5个赤霉病抗性相关QTL,其中1个效应较大的QTL位于2D染色体上,位于标记wmc18-cfd233之间,可解释8.17%~11.42%的表型变异;在3B染色体短臂上检测到1个QTL,位于标记barc102-gwm533之间,可解释5.33%~42.96%的表型变异。QFhb.jaas-2DS与QFhb.jaas-3BS聚合可显著增强小麦赤霉病抗性。另外3个QTL贡献率小于10%,分别位于染色体2B、3B、4A上。检测到与小穗密度相关的QTL有1个,位于3B染色体上,可解释5.36%~6.08%的表型变异。检测到与株高相关的QTL有5个,分别位于染色体4A、7A、5B、6B上,可解释5.2%~8.93%的表型变异。小穗密度与赤霉病抗性呈正相关,株高与抗扩展抗性无相关性,与抗侵染抗性呈负相关。结合以上QTL检测及相关性分析结果可知,QFhb.jaas-3BL可能不是赤霉病抗性位点。因此,包括QFhb.jaas-3BL在内的贡献率小于10%且仅在单一环境下检测到的3个赤霉病抗性相关QTL需进一步进行多年多点试验。
In order to find out the new genes for resistance to Fusarium head blight, we constructed a recombinant inbred line (RILs) containing 184 lines constructed from resistant germplasm N553 and Yangmai 13 using 217 pairs of polymorphisms between parents The QTLs for spikelet density, plant height and scab resistance were analyzed by using this map. The correlation between spikelet density and plant height and scab resistance was also analyzed. The results showed that five QTLs for Fusarium head blight resistance were detected in this study. Among them, one QTL with large effect was located on chromosome 2D and located between markers wmc18-cfd233, which could account for 8.17% ~ 11.42% of phenotypic variation One QTL was detected on the short arm of chromosome 3B, located between the markers barc102-gwm533, which explained 5.33% -42.96% of phenotypic variation. The polymerization of QFhb.jaas-2DS and QFhb.jaas-3BS can significantly enhance the resistance of wheat scab. The other three QTLs contribute less than 10%, located on chromosomes 2B, 3B and 4A, respectively. One QTL associated with spikelet density was detected on chromosome 3B, accounting for 5.36% -6.08% of phenotypic variation. Five QTLs related to plant height were detected on chromosomes 4A, 7A, 5B and 6B, respectively, which explained 5.2% ~ 8.93% of phenotypic variation. There was a positive correlation between spikelet density and scab resistance, no correlation between plant height and resistance to expansion, and negative correlation with resistance to infection. Combined with the above QTL detection and correlation analysis results, QFhb.jaas-3BL may not be scab resistance sites. As a result, three QTLs associated with Fusarium head blight resistance, including QFhb.jaas-3BL, which contributed less than 10% and were detected in only one single environment, required further multi-year multipoint trials.