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以国家种质中期库提供的300份大豆、100份菜豆种质为材料,分别采用血清学和分子生物学方法,对种传病毒的种类进行了检测。结果表明:在大豆种质中检测出大豆花叶病毒(SMV)、黄瓜花叶病毒(CMV)、苜蓿花叶病毒(AMV)3种病毒,阳性检出率分别为25.33%(76份)、13.67%(41份)和4.67%(14份)。大豆种质中还存在大豆花叶病毒与黄瓜花叶病毒、大豆花叶病毒与苜蓿花叶病毒的复合侵染。在菜豆种质中检测出菜豆普通花叶病毒(BCMV)阳性材料92份,种质带毒率高达92%。这些信息将会对今后采取相关措施提高国家种质库保存的豆类种质的质量提供帮助。
300 kinds of soybeans and 100 kinds of kidney bean germplasm supplied from the National Medium Bank were used as material to detect the species of the virus by serological and molecular biological methods respectively. The results showed that the positive detection rates of three viruses (SMV, CMV and AMV) in soybean germplasm were 25.33% (76) 13.67% (41) and 4.67% (14). Soybean germplasm also exists in soybean mosaic virus and cucumber mosaic virus, soybean mosaic virus and alfalfa mosaic virus infection. In common bean germplasm, 92 common bean mosaic virus (BCMV) positive materials were detected, with the germ-banding rate as high as 92%. This information will help to take measures in the future to improve the quality of legume germplasm preserved in the national genebank.