论文部分内容阅读
目的监测杭州白马湖水域鲫鱼的沙门菌感染和耐药状况,了解该水域的水质污染状况。方法收集不同水产市场的244份鲫鱼肠道样本,野生(白马湖水域)样本和健康饲养(养殖场)样本分别为97份和147份,PCR检测沙门菌保守基因inv A与耐药相关基因。K-B药敏纸片法检测细菌的耐药性。结果标本共计检测到6份肠炎沙门菌阳性。鲫鱼沙门菌感染率平均为2.5%(6/244),野生鲫鱼和饲养鲫鱼的感染率分别为3.1%(3/97)和2.0%(3/147)。6株菌均扩增出耐药相关基因par C和gyr A,1株还扩增出了bla TEM和tet B基因。药敏试验显示部分菌呈多重耐药,所有菌对喹诺酮类药物有不同程度耐药,部分菌对β-内酰胺类、氨基糖苷类或四环素类药物耐药。结论野生鲫鱼与饲养鲫鱼均存在肠炎沙门菌感染。药敏试验结果显示,野生鲫鱼耐药程度强于饲养鲫鱼,提示白马湖水域可能存在抗生素污染。
Objective To monitor Salmonella infection and drug resistance of crucian carp in Baima Lake, Hangzhou, and to understand the water pollution in this area. Methods A total of 244 samples of Carassius auratus collected from different aquaculture markets were collected from wild (Baimahu waters) samples and healthy farms (97 farms) and 147 samples respectively. The invA gene of Salmonella typhimurium and resistance-related genes were detected by PCR. K-B drug-sensitive disk method for detecting bacterial resistance. Results A total of 6 specimens of Salmonella enteritidis were detected positive. The average infection rate of Salmonella in crucian carp was 2.5% (6/244), and the infection rates in wild and reared crucian carp were 3.1% (3/97) and 2.0% (3/147) respectively. All six strains amplified the genes related to resistance to par c and gyr A, and one strain also amplified bla TEM and tet B genes. Susceptibility tests showed that some bacteria were multi-drug resistant. All the bacteria had different resistance to quinolones, and some bacteria were resistant to β-lactams, aminoglycosides or tetracyclines. Conclusion Salmonella enteritidis infection is found in both wild and reared crucian carp. Susceptibility test results showed that the resistance of wild crucian carp was higher than that of carp, suggesting that there may be antibiotic contamination in the waters of Baima Lake.